Probe stiffness regulates receptor-ligand bond lifetime under force
文献类型：期刊论文
| 作者 | Zhang Y(章燕) ; Lv SQ(吕守芹); Long M(龙勉)
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| 刊名 | Science China-Physics Mechanics & Astronomy
 |
| 出版日期 | 2011 |
| 卷号 | 54期号:5页码:923-929 |
| 通讯作者邮箱 | mlong@imech.ac.cn |
| ISSN号 | 1674-7348 |
| 关键词 | Probe Stiffness Bond Lifetime Optical Trap Selectin Glycoprotein Ligand-1 P-Selectin Cell-Adhesion Binding-Kinetics Leukocytes Molecule Psgl-1 Flow |
| 通讯作者 | Long, MA (reprint author), Chinese Acad Sci, Inst Mech, Key Lab Micrograv, Beijing 100190, Peoples R China |
| 产权排序 | [Zhang Yan; Lue ShouQin; Long Mian] Chinese Acad Sci, Inst Mech, Key Lab Micrograv, Beijing 100190, Peoples R China; [Zhang Yan; Lue ShouQin; Long Mian] Chinese Acad Sci, Inst Mech, Natl Micrograv Lab, Beijing 100190, Peoples R China; [Zhang Yan; Lue ShouQin; Long Mian] Chinese Acad Sci, Inst Mech, Ctr Biomech & Bioengn, Beijing 100190, Peoples R China |
| 中文摘要 | Receptor-ligand bond dissociation under applied force is crucial to elucidate its biological functionality when the molecular bond is usually connected to a mechanical probe. While the impact of probe stiffness, k, on bond rupture force has recently attracted more and more attention, the mechanism of how it affects the bond lifetime, however, remains unclear. Here we quantified the dissociation lifetime of selectin-ligand bond using an optical trap assay with low stiffness ranging from 3.5x10(-3) to 4.7x10(-2) pN/nm. Our results indicated that bond lifetime yielded distinct distributions with different probe stiffness, implying the stochastic feature of bond dissociation. It was also found that the mean lifetime varied with probe stiffness and that the catch bond nature was visualized at k >= 3.0x10(-2) pN/nm. This work furthered the understanding of the forced dissociation of selectin-ligand bond at varied probe stiffness, which is physiologically relevant to the tethered rolling of leukocytes under blood flow. |
| 学科主题 | Physics |
| 类目[WOS] | Physics, Multidisciplinary |
| 分类号 | 二类/Q3 |
| 研究领域[WOS] | Physics |
| 关键词[WOS] | SELECTIN GLYCOPROTEIN LIGAND-1 ; P-SELECTIN ; CELL-ADHESION ; BINDING-KINETICS ; LEUKOCYTES ; MOLECULE ; PSGL-1 ; FLOW |
| 收录类别 | SCI ; EI |
| 原文出处 | http://dx.doi.org/10.1007/s11433-011-4302-4 |
| 语种 | 英语 |
| WOS记录号 | WOS:000290768300022 |
| 公开日期 | 2012-04-01 |
| 源URL | [http://dspace.imech.ac.cn/handle/311007/45085]  |
| 专题 | 力学研究所_国家微重力实验室 |
| 推荐引用方式 GB/T 7714 | Zhang Y,Lv SQ,Long M. Probe stiffness regulates receptor-ligand bond lifetime under force[J]. Science China-Physics Mechanics & Astronomy,2011,54(5):923-929. |
| APA | 章燕,吕守芹,&龙勉.(2011).Probe stiffness regulates receptor-ligand bond lifetime under force.Science China-Physics Mechanics & Astronomy,54(5),923-929. |
| MLA | 章燕,et al."Probe stiffness regulates receptor-ligand bond lifetime under force".Science China-Physics Mechanics & Astronomy 54.5(2011):923-929. |
入库方式： OAI收割
来源：力学研究所
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