Fluid Dynamics Analysis of a Novel Micropatterned Cell Bioreactor
文献类型：期刊论文
| 作者 | Cui YH; Huo B(霍波) ; Sun SJ(孙树津); Yang F(杨帆); Gao YX(高宇欣); Pan J; Long M(龙勉)
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| 刊名 | Annals of Biomedical Engineering
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| 出版日期 | 2011 |
| 卷号 | 39期号:5页码:1592-1605 |
| 通讯作者邮箱 | mlong@imech.ac.cn |
| 关键词 | Fluid Dynamics Cell Bioreactor Micropattern Steady Shear-Stress Endothelial-Cells Flow Mechanotransduction Environment Prediction Scaffolds Adhesion Chamber Systems |
| ISSN号 | 0090-6964 |
| 产权排序 | [Huo, Bo; Sun, Shujin; Yang, Fan; Gao, Yuxin; Long, Mian] Chinese Acad Sci, Inst Mech, Key Lab Micrograv, Beijing 100190, Peoples R China; [Cui, Yuhong] Tianjin Univ, Dept Mech, Tianjin 300072, Peoples R China; [Huo, Bo; Sun, Shujin; Yang, Fan; Gao, Yuxin; Long, Mian] Natl Micrograv Lab, Beijing 100190, Peoples R China; [Huo, Bo; Sun, Shujin; Yang, Fan; Gao, Yuxin; Long, Mian] Chinese Acad Sci, Inst Mech, Ctr Biomech & Bioengn, Beijing 100190, Peoples R China; [Pan, Jun] Chongqing Univ, Bioengn Coll, Chongqing 400030, Peoples R China |
| 通讯作者 | Long, MA (reprint author), Chinese Acad Sci, Inst Mech, Key Lab Micrograv, Beijing 100190, Peoples R China |
| 合作状况 | 国内 |
| 中文摘要 | Although flow-based bioreactor has been widely used to provide sufficient mass transportation and nutrient supply for cell proliferation, differentiation, and apoptosis, the underlying mechanism of cell responses to applied flow at single cell level remains unclear. This study has developed a novel bioreactor that combines flow bioreactor with microfabrication technique to isolate individual cells onto micropatterned substrate. A mechanical model has also been developed to quantify the flow field or the microenvironment around the single cell; flow dynamics has been analyzed on five geometrically different patterns of circle-, cube-, 1:2 ellipse-, 1:3 ellipse-, and rectangle-shaped "virtual cells." The results of this study have demonstrated that the flow field is highly pattern dependent, and all the hydrodynamic development length, cell spacing, and orientation of inlet velocity vector are crucial for maintaining a fully developed flow. This study has provided a theoretical basis for optimizing the design of micropatterned flow bioreactor and a novel approach to understand the cell mechanotransduction and cell-surface interaction at single cell level. |
| 学科主题 | Engineering |
| 分类号 | 二类/Q2 |
| 类目[WOS] | Engineering, Biomedical |
| 研究领域[WOS] | Engineering |
| 关键词[WOS] | STEADY SHEAR-STRESS ; ENDOTHELIAL-CELLS ; FLOW ; MECHANOTRANSDUCTION ; ENVIRONMENT ; PREDICTION ; SCAFFOLDS ; ADHESION ; CHAMBER ; SYSTEMS |
| 收录类别 | SCI ; EI |
| 资助信息 | The authors are grateful to Xin Wang, Yunfeng Wu, and Yabin Zhai for computational assistance. This study was supported by the following grants: the Natural Science Foundation of China, #30730032 and #30870606; Knowledge Innovation Program of CAS, #KJCX2-YW-L08; the National Key Basic Research Foundation of China, #2006CB910303; and the National High Technology Research and Development Program of China, #2007AA02Z306. |
| 原文出处 | http://dx.doi.org/10.1007/s10439-011-0250-4 |
| 语种 | 英语 |
| WOS记录号 | WOS:000289243100020 |
| 公开日期 | 2012-04-01 |
| 源URL | [http://dspace.imech.ac.cn/handle/311007/45086]  |
| 专题 | 力学研究所_国家微重力实验室 |
| 推荐引用方式 GB/T 7714 | Cui YH,Huo B,Sun SJ,et al. Fluid Dynamics Analysis of a Novel Micropatterned Cell Bioreactor[J]. Annals of Biomedical Engineering,2011,39(5):1592-1605. |
| APA | Cui YH.,霍波.,孙树津.,杨帆.,高宇欣.,...&龙勉.(2011).Fluid Dynamics Analysis of a Novel Micropatterned Cell Bioreactor.Annals of Biomedical Engineering,39(5),1592-1605. |
| MLA | Cui YH,et al."Fluid Dynamics Analysis of a Novel Micropatterned Cell Bioreactor".Annals of Biomedical Engineering 39.5(2011):1592-1605. |
入库方式： OAI收割
来源：力学研究所
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