Two kinds of anti-ricin antibody and ricin interaction evaluated by biosensor based on imaging ellipsometry
文献类型：期刊论文
| 作者 | Niu Y(牛宇) ; Zhuang J; Liu L(刘丽); Yan XY; Jin G(靳刚)
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| 刊名 | Thin Solid Films
 |
| 出版日期 | 2011 |
| 卷号 | 519期号:9页码:2768-2771 |
| 通讯作者邮箱 | gajin@imech.ac.cn |
| 关键词 | Ricin Detection Antigen-Antibody Interaction Biosensor Imaging Ellipsometry Neutralizing Monoclonal-Antibody Optical Protein-Chip Silicon Surface Immobilization Visualization System Cells Assay |
| ISSN号 | 0040-6090 |
| 产权排序 | [Niu, Yu; Liu, Li; Jin, Gang] Chinese Acad Sci, NML, Inst Mech, Beijing 100190, Peoples R China; [Niu, Yu] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China; [Zhuang, Jie; Yan, Xiyun] Chinese Acad Sci, Inst Biophys, Beijing 100101, Peoples R China |
| 通讯作者 | Jin, G (reprint author), Chinese Acad Sci, NML, Inst Mech, 15 Bei Si Huan W Rd, Beijing 100190, Peoples R China |
| 合作状况 | 国内 |
| 中文摘要 | In order to meet the requirements of antibody comparison and evaluation, the optimization for the surface modification, ligand immobilization, unspecific blocking and ligand receptor interaction condition is introduced to the biosensor based on imaging ellipsometry (BIE), so that it may serve as an antibody screening platform to evaluate antibody antigen interactions. Two kinds of anti-ricin antibody named pVHHS1 and 5S1R are immobilized in a patterned array format on the silicon surface modified with succinic anhydride, respectively, to form a sensing surface. Ricin and its similar toxin abrin as the reference in solution are delivered, respectively, on the patterned sensing surface for anti-ricin antibody and ricin interaction. The limit of detection value of ricin detected by pVHHS1 and 5S1R is obtained as 1 mu g/ml and 5 ng/ml, respectively. Results show that pVHHS1 and 5S1R specifically bind with ricin but not with abrin, which is in accordance with ELISA's result. (C) 2010 Elsevier B.V. All rights reserved. |
| 学科主题 | Materials Science; Physics |
| 分类号 | 二类/Q2 |
| 类目[WOS] | Materials Science, Multidisciplinary ; Materials Science, Coatings & Films ; Physics, Applied ; Physics, Condensed Matter |
| 研究领域[WOS] | Materials Science ; Physics |
| 关键词[WOS] | NEUTRALIZING MONOCLONAL-ANTIBODY ; OPTICAL PROTEIN-CHIP ; SILICON SURFACE ; IMMOBILIZATION ; VISUALIZATION ; SYSTEM ; CELLS ; ASSAY |
| 收录类别 | SCI ; EI |
| 原文出处 | http://dx.doi.org/10.1016/j.tsf.2010.12.054 |
| 语种 | 英语 |
| WOS记录号 | WOS:000289174200043 |
| 公开日期 | 2012-04-01 |
| 源URL | [http://dspace.imech.ac.cn/handle/311007/45102]  |
| 专题 | 力学研究所_国家微重力实验室 |
| 推荐引用方式 GB/T 7714 | Niu Y,Zhuang J,Liu L,et al. Two kinds of anti-ricin antibody and ricin interaction evaluated by biosensor based on imaging ellipsometry[J]. Thin Solid Films,2011,519(9):2768-2771. |
| APA | 牛宇,Zhuang J,刘丽,Yan XY,&靳刚.(2011).Two kinds of anti-ricin antibody and ricin interaction evaluated by biosensor based on imaging ellipsometry.Thin Solid Films,519(9),2768-2771. |
| MLA | 牛宇,et al."Two kinds of anti-ricin antibody and ricin interaction evaluated by biosensor based on imaging ellipsometry".Thin Solid Films 519.9(2011):2768-2771. |
入库方式： OAI收割
来源：力学研究所
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