A three-layer PMMA electrophoresis microchip with Pt microelectrodes insulated by a thin film for contactless conductivity detection
文献类型：期刊论文
| 作者 | Liu JS; Wang JY; Chen ZG; Yu Y(于泳) ; Yang XJ; Zhang XB; Xu Z
|
| 刊名 | Lab on A Chip
 |
| 出版日期 | 2011 |
| 卷号 | 11期号:5页码:969-973 |
| 通讯作者邮箱 | liujs@dlut.edu.cn; chenzg@mail.sysu.edu.cn; yuyong@imech.ac.cn |
| 关键词 | Capillary-Electrophoresis Fabrication System Polymerization Electrodes Devices Chip |
| ISSN号 | 1473-0197 |
| 产权排序 | [Liu, Junshan; Wang, Junyao; Zhang, Xianbin; Xu, Zheng; Liu, Chong] Dalian Univ Technol, Key Lab Micro Nano Technol & Syst Liaoning Prov, Dalian 116023, Liaoning, Peoples R China; [Chen, Zuanguang; Yang, Xiujuan] Sun Yat Sen Univ, Sch Pharmaceut Sci, Guangzhou 510089, Guangdong, Peoples R China; [Yu, Yong] Chinese Acad Sci, Inst Mech, Natl Micrograv Lab, Beijing 100190, Peoples R China |
| 通讯作者 | Liu, JS (reprint author), Dalian Univ Technol, Key Lab Micro Nano Technol & Syst Liaoning Prov, Dalian 116023, Liaoning, Peoples R China |
| 中文摘要 | A three-layer poly (methyl methacrylate) (PMMA) electrophoresis microchip integrated with Pt microelectrodes for contactless conductivity detection is presented. A 50 mu m-thick PMMA film is used as the insulating layer and placed between the channel plate (containing the microchannel) and the electrode plate (containing the microelectrode). The three-layer structure facilitates the achievement of a thin insulating layer, obviates the difficulty of integrating microelectrodes on a thin film, and does not compromise the integration of microchips. To overcome the thermal and chemical incompatibilities of polymers and photolithographic techniques, a modified lift-off process was developed to integrate Pt microelectrodes onto the PMMA substrate. A novel two-step bonding method was created to assemble the complete PMMA microchip. A low limit of detection of 1.25 mu g ml(-1) for Na(+) and high separation efficiency of 77 000 and 48 000 plates/m for Na(+) and K(+) were obtained when operating the detector at a low excitation frequency of 60 kHz. |
| 学科主题 | Biochemistry & Molecular Biology; Chemistry; Science & Technology - Other Topics |
| 分类号 | 一类 |
| 类目[WOS] | Biochemical Research Methods ; Chemistry, Multidisciplinary ; Nanoscience & Nanotechnology |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Chemistry ; Science & Technology - Other Topics |
| 关键词[WOS] | CAPILLARY-ELECTROPHORESIS ; FABRICATION ; SYSTEM ; POLYMERIZATION ; ELECTRODES ; DEVICES ; CHIP |
| 收录类别 | SCI |
| 资助信息 | This work was supported by the National Natural Science Foundation of China (50605006, 51075056, 50705012, 20890024, 20727006), and the National Basic Research Program of China (2007CB714502). We thank Yanan Du, Jason W. Nichol and Seila Selimovic for scientific discussions and revisions. J. Liu, Y. Yu, Z. Chen, Z. Xu and C. Liu designed the research strategy; J. Liu, J. Wang, X. Yang and X. Zhang performed the experiments; Y. Yu designed the microchip; J. Liu, J. Wang and X. Yang wrote the paper. |
| 原文出处 | http://dx.doi.org/10.1039/c0lc00341g |
| 语种 | 英语 |
| WOS记录号 | WOS:000287409600028 |
| 公开日期 | 2012-04-01 |
| 源URL | [http://dspace.imech.ac.cn/handle/311007/45108]  |
| 专题 | 力学研究所_国家微重力实验室 |
| 推荐引用方式 GB/T 7714 | Liu JS,Wang JY,Chen ZG,et al. A three-layer PMMA electrophoresis microchip with Pt microelectrodes insulated by a thin film for contactless conductivity detection[J]. Lab on A Chip,2011,11(5):969-973. |
| APA | Liu JS.,Wang JY.,Chen ZG.,Yu Y.,Yang XJ.,...&Xu Z.(2011).A three-layer PMMA electrophoresis microchip with Pt microelectrodes insulated by a thin film for contactless conductivity detection.Lab on A Chip,11(5),969-973. |
| MLA | Liu JS,et al."A three-layer PMMA electrophoresis microchip with Pt microelectrodes insulated by a thin film for contactless conductivity detection".Lab on A Chip 11.5(2011):969-973. |
入库方式： OAI收割
来源：力学研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。