Approach to Quantitative Detection of CD146 with the Label-free Protein Biosensor Based on Imaging Ellipsometry
文献类型：会议论文
| 作者 | Niu Y(牛宇) ; Liu L(刘丽); Yan XY; Jin G(靳刚)
|
| 出版日期 | 2011 |
| 会议名称 | Conference on Sensors, Cameras, and Systems for Industrial, Scientific, and Consumer Applications XII |
| 会议日期 | JAN 25-27, 2011 |
| 会议地点 | San Francisco, CA |
| 通讯作者邮箱 | gajin@imech.ac.cn |
| 关键词 | CD146 detection biosensor imaging ellipsometry MEL-CAM ANTIBODY VISUALIZATION GROWTH CANCER |
| 卷号 | 7875 |
| 页码 | 78750R |
| 通讯作者 | Jin, G (reprint author), Chinese Acad Sci, NML, Inst Mech, 15 Bei Si Huan W Rd, Beijing 100190, Peoples R China |
| 中文摘要 | CD146 glycoprotein belonging to cell adhesion molecules is considered to be a novel target on endothelial cell involved in tumor angiogenesis. The biosensor based on imaging ellipsometry (BIE) which is performed in null and off-null mode is used for CD146 detection as a trial by the following steps. Firstly, anti-CD146 antibody as ligand is immobilized on Protein G modified silicon substrate. Then, CD146 test is carried out and its calibration curve is established for the requirement of quantitative detection. Finally, 18 serum samples are detected quantitatively and their results are validated by ELISA's. The sensitivity for CD146 detection achieves the order of ng/ml and the relationship between BIE signal y (grayscale value) and CD146 concentration x (ng/ml) is y=3.3ln(x) +91.3. Compared with ELISA's, the majority of results are in agreement, and the results of two approaches have significant statistic relevance. |
| 收录类别 | CPCI-S |
| 合作状况 | 国内 |
| 产权排序 | Chinese Acad Sci, NML, Inst Mech, Beijing 100190, Peoples R China |
| 会议网址 | http://dx.doi.org/10.1117/12.871972 |
| 会议录 | SENSORS, CAMERAS, AND SYSTEMS FOR INDUSTRIAL, SCIENTIFIC, AND CONSUMER APPLICATIONS XII
 |
| 会议录出版者 | SPIE-INT SOC OPTICAL ENGINEERING |
| 学科主题 | Optics |
| 会议录出版地 | 1000 20TH ST, PO BOX 10, BELLINGHAM, WA 98227-0010 USA |
| 语种 | 英语 |
| ISSN号 | 0277-786X |
| ISBN号 | 978-0-8194-8412-3 |
| WOS记录号 | WOS:000299998500249 |
| 源URL | [http://dspace.imech.ac.cn/handle/311007/45315]  |
| 专题 | 力学研究所_国家微重力实验室 |
| 推荐引用方式 GB/T 7714 | Niu Y,Liu L,Yan XY,et al. Approach to Quantitative Detection of CD146 with the Label-free Protein Biosensor Based on Imaging Ellipsometry[C]. 见:Conference on Sensors, Cameras, and Systems for Industrial, Scientific, and Consumer Applications XII. San Francisco, CA. JAN 25-27, 2011.http://dx.doi.org/10.1117/12.871972. |
入库方式： OAI收割
来源：力学研究所
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