Improvement for sensitivity of biosensor with total internal reflection imaging ellipsometry (TIRIE)
文献类型：会议论文
| 作者 | Liu L(刘丽) ; Chen YY(陈艳艳); Meng YH(孟永宏); Chen S(陈涉); Jin G(靳刚)
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| 出版日期 | 2011 |
| 会议名称 | 5th International Conference on Spectroscopic Ellipsometry |
| 会议日期 | MAY 23-29, 2010 |
| 会议地点 | Albany, NY |
| 通讯作者邮箱 | gajin@imech.ac.cn |
| 关键词 | Sensitivity improvement Imaging ellipsometry Total internal reflection Biosensor HbsAg detection |
| 卷号 | 519 |
| 期号 | 9 |
| 页码 | 2758-2762 |
| 通讯作者 | Jin, G (reprint author), Chinese Acad Sci, Natl Micrograv Lab, Inst Mech, 15 Bei Si Huan W Rd, Beijing 100190, Peoples R China |
| 中文摘要 | The biosensor based on the total internal reflection imaging ellipsometry (TIRIE) is realized as an automatic analysis method for protein interaction processes in real-time, with high throughput and label-free. An evanescent wave is used as the optical probe to monitor bio-molecular interactions on a chip surface with a high sensitivity due to its phase sensitive property. In this paper, the technique is optimized with a polarization setting, a spectroscopic light source and a low noise CCD detector to improve the performance of the biosensor in sensitivity and detection limit, as evidenced by a quantitative detection of Hepatitis B virus surface antigen (HbsAg) with concentrations of 8, 16, 32, 64, 125 and 250 ng/ml. The sensitivity is increased by one order of magnitude and the detection limit has been extended more than 50 times for HbsAg detection. (C) 2011 Elsevier B.V. All rights reserved. |
| 收录类别 | CPCI |
| 合作状况 | 国内 |
| 产权排序 | Chinese Acad Sci, Natl Micrograv Lab, Inst Mech, Beijing 100190, Peoples R China; Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China; Chinese Acad Sci, Inst Nanotech & Nanobion, Suzhou 215125, Peoples R China |
| 会议主办者 | Univ Albany, Coll Nanoscale & Engn |
| 会议网址 | http://dx.doi.org/10.1016/j.tsf.2010.12.130 |
| 会议录 | THIN SOLID FILMS
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| 会议录出版者 | ELSEVIER SCIENCE SA |
| 学科主题 | Materials Science, Multidisciplinary; Materials Science, Coatings & Films; Physics, Applied; Physics, Condensed Matter |
| 会议录出版地 | PO BOX 564, 1001 LAUSANNE, SWITZERLAND |
| 语种 | 英语 |
| ISSN号 | 0040-6090 |
| 源URL | [http://dspace.imech.ac.cn/handle/311007/45319]  |
| 专题 | 力学研究所_国家微重力实验室 |
| 推荐引用方式 GB/T 7714 | Liu L,Chen YY,Meng YH,et al. Improvement for sensitivity of biosensor with total internal reflection imaging ellipsometry (TIRIE)[C]. 见:5th International Conference on Spectroscopic Ellipsometry. Albany, NY. MAY 23-29, 2010.http://dx.doi.org/10.1016/j.tsf.2010.12.130. |
入库方式： OAI收割
来源：力学研究所
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