Probe stiffness regulates receptor-ligand bond lifetime under force
文献类型：会议论文
| 作者 | Lv SQ(吕守芹) ; Zhang Y(章燕); Long M(龙勉)
|
| 出版日期 | 2011 |
| 会议名称 | Symposium on Multiscale Modeling and Simulation of Materials/11th International Conference in Asia of the International-Union-of-Materials-Research-Societies |
| 会议日期 | SEP 25-28, 2010 |
| 会议地点 | Qingdao, PEOPLES R CHINA |
| 通讯作者邮箱 | mlong@imech.ac.cn |
| 关键词 | probe stiffness bond lifetime optical trap |
| 卷号 | 54 |
| 期号 | 5 |
| 页码 | 923-929 |
| 通讯作者 | Long, MA (reprint author), Chinese Acad Sci, Inst Mech, Key Lab Micrograv, Beijing 100190, Peoples R China |
| 中文摘要 | Receptor-ligand bond dissociation under applied force is crucial to elucidate its biological functionality when the molecular bond is usually connected to a mechanical probe. While the impact of probe stiffness, k, on bond rupture force has recently attracted more and more attention, the mechanism of how it affects the bond lifetime, however, remains unclear. Here we quantified the dissociation lifetime of selectin-ligand bond using an optical trap assay with low stiffness ranging from 3.5x10(-3) to 4.7x10(-2) pN/nm. Our results indicated that bond lifetime yielded distinct distributions with different probe stiffness, implying the stochastic feature of bond dissociation. It was also found that the mean lifetime varied with probe stiffness and that the catch bond nature was visualized at k >= 3.0x10(-2) pN/nm. This work furthered the understanding of the forced dissociation of selectin-ligand bond at varied probe stiffness, which is physiologically relevant to the tethered rolling of leukocytes under blood flow. |
| 收录类别 | CPCI（ISTP） |
| 产权排序 | Chinese Acad Sci, Inst Mech, Key Lab Micrograv, Beijing 100190, Peoples R China; Chinese Acad Sci, Inst Mech, Natl Micrograv Lab, Beijing 100190, Peoples R China; Chinese Acad Sci, Inst Mech, Ctr Biomech & Bioengn, Beijing 100190, Peoples R China |
| 会议网址 | http://dx.doi.org/10.1007/s11433-011-4302-4 |
| 会议录 | SCIENCE CHINA-PHYSICS MECHANICS & ASTRONOMY
 |
| 会议录出版者 | SCIENCE CHINA PRESS |
| 学科主题 | Physics, Multidisciplinary |
| 会议录出版地 | 16 DONGHUANGCHENGGEN NORTH ST, BEIJING 100717, PEOPLES R CHINA |
| 语种 | 英语 |
| ISSN号 | 1674-7348 |
| 源URL | [http://dspace.imech.ac.cn/handle/311007/45325]  |
| 专题 | 力学研究所_国家微重力实验室 |
| 推荐引用方式 GB/T 7714 | Lv SQ,Zhang Y,Long M. Probe stiffness regulates receptor-ligand bond lifetime under force[C]. 见:Symposium on Multiscale Modeling and Simulation of Materials/11th International Conference in Asia of the International-Union-of-Materials-Research-Societies. Qingdao, PEOPLES R CHINA. SEP 25-28, 2010.http://dx.doi.org/10.1007/s11433-011-4302-4. |
入库方式： OAI收割
来源：力学研究所
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