Two kinds of anti-ricin antibody and ricin interaction evaluated by biosensor based on imaging ellipsometry
文献类型：会议论文
| 作者 | Liu L(刘丽) ; Jin G(靳刚); Niu Y(牛宇)
|
| 出版日期 | 2011 |
| 会议名称 | 5th International Conference on Spectroscopic Ellipsometry |
| 会议日期 | MAY 23-29, 2010 |
| 会议地点 | Albany, NY |
| 通讯作者邮箱 | gajin@imech.ac.cn |
| 关键词 | Ricin detection Antigen-antibody interaction Biosensor Imaging ellipsometry |
| 卷号 | 519 |
| 期号 | 9 |
| 页码 | 2768-2771 |
| 通讯作者 | Jin, G (reprint author), Chinese Acad Sci, NML, Inst Mech, 15 Bei Si Huan W Rd, Beijing 100190, Peoples R China |
| 中文摘要 | In order to meet the requirements of antibody comparison and evaluation, the optimization for the surface modification, ligand immobilization, unspecific blocking and ligand receptor interaction condition is introduced to the biosensor based on imaging ellipsometry (BIE), so that it may serve as an antibody screening platform to evaluate antibody antigen interactions. Two kinds of anti-ricin antibody named pVHHS1 and 5S1R are immobilized in a patterned array format on the silicon surface modified with succinic anhydride, respectively, to form a sensing surface. Ricin and its similar toxin abrin as the reference in solution are delivered, respectively, on the patterned sensing surface for anti-ricin antibody and ricin interaction. The limit of detection value of ricin detected by pVHHS1 and 5S1R is obtained as 1 mu g/ml and 5 ng/ml, respectively. Results show that pVHHS1 and 5S1R specifically bind with ricin but not with abrin, which is in accordance with ELISA's result. (C) 2010 Elsevier B.V. All rights reserved. |
| 收录类别 | CPCI（ISTP） |
| 合作状况 | 国内 |
| 产权排序 | Chinese Acad Sci, NML, Inst Mech, Beijing 100190, Peoples R China; Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China; Chinese Acad Sci, Inst Biophys, Beijing 100101, Peoples R China |
| 会议主办者 | Univ Albany, Coll Nanoscale & Engn |
| 会议网址 | http://dx.doi.org/10.1016/j.tsf.2010.12.054 |
| 会议录 | THIN SOLID FILMS
 |
| 会议录出版者 | ELSEVIER SCIENCE SA |
| 学科主题 | Materials Science, Multidisciplinary; Materials Science, Coatings & Films; Physics, Applied; Physics, Condensed Matter |
| 会议录出版地 | PO BOX 564, 1001 LAUSANNE, SWITZERLAND |
| 语种 | 英语 |
| ISSN号 | 0040-6090 |
| 源URL | [http://dspace.imech.ac.cn/handle/311007/45328]  |
| 专题 | 力学研究所_国家微重力实验室 |
| 推荐引用方式 GB/T 7714 | Liu L,Jin G,Niu Y. Two kinds of anti-ricin antibody and ricin interaction evaluated by biosensor based on imaging ellipsometry[C]. 见:5th International Conference on Spectroscopic Ellipsometry. Albany, NY. MAY 23-29, 2010.http://dx.doi.org/10.1016/j.tsf.2010.12.054. |
入库方式： OAI收割
来源：力学研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。