Evaluation of the Role of Functional Constraints on the Integrity of an Ultraconserved Region in the Genus Drosophila
文献类型：期刊论文
| 作者 | Diaz-Castillo, Carlos1; Xia, Xiao-Qin2; Ranz, Jose M.1 |
| 刊名 | PLOS GENETICS
 |
| 出版日期 | 2012-02-01 |
| 卷号 | 8期号:2页码:e1002475 |
| ISSN号 | 1553-7404 |
| 通讯作者 | Diaz-Castillo, C (reprint author), Univ Calif Irvine, Dept Ecol & Evolutionary Biol, Irvine, CA 92717 USA |
| 中文摘要 | Why gene order is conserved over long evolutionary timespans remains elusive. A common interpretation is that gene order conservation might reflect the existence of functional constraints that are important for organismal performance. Alteration of the integrity of genomic regions, and therefore of those constraints, would result in detrimental effects. This notion seems especially plausible in those genomes that can easily accommodate gene reshuffling via chromosomal inversions since genomic regions free of constraints are likely to have been disrupted in one or more lineages. Nevertheless, no empirical test has been performed to this notion. Here, we disrupt one of the largest conserved genomic regions of the Drosophila genome by chromosome engineering and examine the phenotypic consequences derived from such disruption. The targeted region exhibits multiple patterns of functional enrichment suggestive of the presence of constraints. The carriers of the disrupted collinear block show no defects in their viability, fertility, and parameters of general homeostasis, although their odorant perception is altered. This change in odorant perception does not correlate with modifications of the level of expression and sex bias of the genes within the genomic region disrupted. Our results indicate that even in highly rearranged genomes, like those of Diptera, unusually high levels of gene order conservation cannot be systematically attributed to functional constraints, which raises the possibility that other mechanisms can be in place and therefore the underpinnings of the maintenance of gene organization might be more diverse than previously thought. |
| 英文摘要 | Why gene order is conserved over long evolutionary timespans remains elusive. A common interpretation is that gene order conservation might reflect the existence of functional constraints that are important for organismal performance. Alteration of the integrity of genomic regions, and therefore of those constraints, would result in detrimental effects. This notion seems especially plausible in those genomes that can easily accommodate gene reshuffling via chromosomal inversions since genomic regions free of constraints are likely to have been disrupted in one or more lineages. Nevertheless, no empirical test has been performed to this notion. Here, we disrupt one of the largest conserved genomic regions of the Drosophila genome by chromosome engineering and examine the phenotypic consequences derived from such disruption. The targeted region exhibits multiple patterns of functional enrichment suggestive of the presence of constraints. The carriers of the disrupted collinear block show no defects in their viability, fertility, and parameters of general homeostasis, although their odorant perception is altered. This change in odorant perception does not correlate with modifications of the level of expression and sex bias of the genes within the genomic region disrupted. Our results indicate that even in highly rearranged genomes, like those of Diptera, unusually high levels of gene order conservation cannot be systematically attributed to functional constraints, which raises the possibility that other mechanisms can be in place and therefore the underpinnings of the maintenance of gene organization might be more diverse than previously thought. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Genetics & Heredity |
| 研究领域[WOS] | Genetics & Heredity |
| 关键词[WOS] | CONSERVED NONCODING SEQUENCES ; GLOBAL CONTROL REGION ; P-ELEMENT INSERTIONS ; MICROARRAY DATA ; CHROMOSOMAL REARRANGEMENT ; REGULATORY LANDSCAPES ; MELANOGASTER GENOME ; GENETIC-VARIABILITY ; ANOPHELES-GAMBIAE ; HOXD CLUSTER |
| 收录类别 | SCI |
| 资助信息 | NSF[DEB-0949365] |
| 语种 | 英语 |
| WOS记录号 | WOS:000300725500006 |
| 公开日期 | 2012-04-01 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/16761]  |
| 专题 | 水生生物研究所_水生生物分子与细胞生物学研究中心_期刊论文 |
| 作者单位 | 1.Univ Calif Irvine, Dept Ecol & Evolutionary Biol, Irvine, CA 92717 USA 2.Chinese Acad Sci, Inst Hydrobiol, Wuhan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Diaz-Castillo, Carlos,Xia, Xiao-Qin,Ranz, Jose M.. Evaluation of the Role of Functional Constraints on the Integrity of an Ultraconserved Region in the Genus Drosophila[J]. PLOS GENETICS,2012,8(2):e1002475. |
| APA | Diaz-Castillo, Carlos,Xia, Xiao-Qin,&Ranz, Jose M..(2012).Evaluation of the Role of Functional Constraints on the Integrity of an Ultraconserved Region in the Genus Drosophila.PLOS GENETICS,8(2),e1002475. |
| MLA | Diaz-Castillo, Carlos,et al."Evaluation of the Role of Functional Constraints on the Integrity of an Ultraconserved Region in the Genus Drosophila".PLOS GENETICS 8.2(2012):e1002475. |
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来源：水生生物研究所
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