QUICK identification and SPR validation of signal transducers and activators of transcription 3 (Stat3) interacting proteins
文献类型：期刊论文
| 作者 | Zheng, Peng2; Zhong, Qiu3; Xiong, Qian2; Yang, Mingkun2; Zhang, Jia1,2; Li, Chongyang2; Bi, Li-Jun1; Ge, Feng2 |
| 刊名 | JOURNAL OF PROTEOMICS
 |
| 出版日期 | 2012-01-04 |
| 卷号 | 75期号:3页码:1055-1066 |
| 关键词 | Quantitative immunoprecipitation combined with knockdown (QUICK) Stable isotope labeling with amino acids in cell culture (SILAC) Stat3 14-3-3 zeta Multiple myeloma Surface plasmon resonance (SPR) |
| ISSN号 | 1874-3919 |
| 通讯作者 | Bi, LJ (reprint author), Chinese Acad Sci, Natl Lab Biomacromol, Inst Biophys, Beijing 100101, Peoples R China |
| 中文摘要 | Signal transducers and activators of transcription 3 (Stat3) has been reported to be involved in the pathogenesis of various human diseases and is constitutively active in human multiple myeloma (MM) U266 cells. The Stat3-regulated mechanisms involved in these processes, however, are not fully defined. To further understand the regulation of Stat3 activity, we performed a systematic proteomic analysis of Stat3 interacting proteins in U266 cells. This analysis, termed quantitative immunoprecipitation combined with knockdown (QUICK), combines RNAi, stable isotope labeling with amino acids in cell culture (SILAC), immunoprecipitation, and quantitative MS. As a result, quantitative mass spectrometry analysis allowed us to distinguish specific Stat3 interacting proteins from background proteins and led to the identification of a total of 38 proteins. Three Stat3 interacting proteins - 14-3-3 zeta, PRKCB and Hsp90 - were further confirmed by reciprocal co-immunoprecipitations and surface plasmon resonance (SPR) analysis. Our results therefore not only uncover a number of Stat3 interacting proteins that possess a variety of cellular functions, but also provide new insight into the mechanisms that regulate Stat3 activity and function in MM cells. (C) 2011 Elsevier B.V. All rights reserved. |
| 英文摘要 | Signal transducers and activators of transcription 3 (Stat3) has been reported to be involved in the pathogenesis of various human diseases and is constitutively active in human multiple myeloma (MM) U266 cells. The Stat3-regulated mechanisms involved in these processes, however, are not fully defined. To further understand the regulation of Stat3 activity, we performed a systematic proteomic analysis of Stat3 interacting proteins in U266 cells. This analysis, termed quantitative immunoprecipitation combined with knockdown (QUICK), combines RNAi, stable isotope labeling with amino acids in cell culture (SILAC), immunoprecipitation, and quantitative MS. As a result, quantitative mass spectrometry analysis allowed us to distinguish specific Stat3 interacting proteins from background proteins and led to the identification of a total of 38 proteins. Three Stat3 interacting proteins - 14-3-3 zeta, PRKCB and Hsp90 - were further confirmed by reciprocal co-immunoprecipitations and surface plasmon resonance (SPR) analysis. Our results therefore not only uncover a number of Stat3 interacting proteins that possess a variety of cellular functions, but also provide new insight into the mechanisms that regulate Stat3 activity and function in MM cells. (C) 2011 Elsevier B.V. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biochemical Research Methods |
| 研究领域[WOS] | Biochemistry & Molecular Biology |
| 关键词[WOS] | MULTIPLE-MYELOMA CELLS ; CONSTITUTIVE ACTIVATION ; PHOSPHATASE 2A ; KINASE-C ; IN-VIVO ; PROSTATE-CANCER ; KNOCKDOWN QUICK ; GENE ONTOLOGY ; TARGETING PKC ; APOPTOSIS |
| 收录类别 | SCI |
| 资助信息 | Chinese Academy of Sciences; National Basic Research Program of China (973 Program)[2012CB518700]; National Laboratory of Biomacromolecules |
| 语种 | 英语 |
| WOS记录号 | WOS:000299719900027 |
| 公开日期 | 2012-04-01 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/16763]  |
| 专题 | 水生生物研究所_水生生物分子与细胞生物学研究中心_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Natl Lab Biomacromol, Inst Biophys, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China 3.AntiTB Res Inst Guangdong Prov, IBP ARI Joint Ctr Res TB, Guangzhou 510630, Guangdong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zheng, Peng,Zhong, Qiu,Xiong, Qian,et al. QUICK identification and SPR validation of signal transducers and activators of transcription 3 (Stat3) interacting proteins[J]. JOURNAL OF PROTEOMICS,2012,75(3):1055-1066. |
| APA | Zheng, Peng.,Zhong, Qiu.,Xiong, Qian.,Yang, Mingkun.,Zhang, Jia.,...&Ge, Feng.(2012).QUICK identification and SPR validation of signal transducers and activators of transcription 3 (Stat3) interacting proteins.JOURNAL OF PROTEOMICS,75(3),1055-1066. |
| MLA | Zheng, Peng,et al."QUICK identification and SPR validation of signal transducers and activators of transcription 3 (Stat3) interacting proteins".JOURNAL OF PROTEOMICS 75.3(2012):1055-1066. |
入库方式： OAI收割
来源：水生生物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。