Optimal strategies for determination of free/extractable and total microcystins in lake sediment
文献类型：期刊论文
| 作者 | Wu, Xingqiang2; Wang, Chunbo1,3; Xiao, Bangding1; Wang, Yang2; Zheng, Na2; Liu, Jingshuang2 |
| 刊名 | ANALYTICA CHIMICA ACTA
 |
| 出版日期 | 2012-01-04 |
| 卷号 | 709期号:0页码:66-72 |
| 关键词 | Microcystin 2-Methyl-3-methoxy-4-phenylbutyric acid (MMPB) Sediment Solvent extraction Lemieux oxidation |
| ISSN号 | 0003-2670 |
| 通讯作者 | Xiao, BD (reprint author), Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China |
| 中文摘要 | The optimization of analytical procedures for the quantification of free and total microcystins (MCs) in natural sediments was systematically examined based on solvent extraction and Lemieux oxidation. In this optimized analytical procedure, a sequential solvent extraction using 50% (v/v) methanol and EDTA-sodium pyrophosphate was selected as the optimal extraction solvent for free MCs analysis, after which the purified extracts and sediment residuals were applied to the optimized Lemieux oxidation for determination of total MCs in lake sediments. The optimized procedures were shown to be efficient and reliable for the routine analysis of both free and total MCs in lake sediment samples, as indicated by the minimal adverse impact of sediment organic matter on the recovery of free MCs and yield of MMPB (2-methyl-3-methoxy-4-phenylbutyric acid). Finally, the developed procedures were applied to field sediment samples collected from Lake Dianchi during a bloom season and seven of thirty samples showed positive results. (C) 2011 Elsevier B.V. All rights reserved. |
| 英文摘要 | The optimization of analytical procedures for the quantification of free and total microcystins (MCs) in natural sediments was systematically examined based on solvent extraction and Lemieux oxidation. In this optimized analytical procedure, a sequential solvent extraction using 50% (v/v) methanol and EDTA-sodium pyrophosphate was selected as the optimal extraction solvent for free MCs analysis, after which the purified extracts and sediment residuals were applied to the optimized Lemieux oxidation for determination of total MCs in lake sediments. The optimized procedures were shown to be efficient and reliable for the routine analysis of both free and total MCs in lake sediment samples, as indicated by the minimal adverse impact of sediment organic matter on the recovery of free MCs and yield of MMPB (2-methyl-3-methoxy-4-phenylbutyric acid). Finally, the developed procedures were applied to field sediment samples collected from Lake Dianchi during a bloom season and seven of thirty samples showed positive results. (C) 2011 Elsevier B.V. All rights reserved. |
| WOS标题词 | Science & Technology ; Physical Sciences |
| 类目[WOS] | Chemistry, Analytical |
| 研究领域[WOS] | Chemistry |
| 关键词[WOS] | COVALENTLY BOUND MICROCYSTINS ; LIQUID-CHROMATOGRAPHY ; RISK-ASSESSMENT ; ANIMAL-TISSUES ; LR ; WATER ; CYANOBACTERIA ; EXTRACTION ; BIOACCUMULATION ; OPTIMIZATION |
| 收录类别 | SCI |
| 资助信息 | National Natural Science Foundation of China[41001326]; National Key Project for Basic Research[2008CB418101]; Chinese Academy of Sciences[KZCX2-YW-QN306] |
| 语种 | 英语 |
| WOS记录号 | WOS:000299801500008 |
| 公开日期 | 2012-04-01 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/16749]  |
| 专题 | 水生生物研究所_水环境工程研究中心_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China 2.Chinese Acad Sci, NE Inst Geog & Agroecol, Key Lab Wetland Ecol & Environm, Changchun 130012, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wu, Xingqiang,Wang, Chunbo,Xiao, Bangding,et al. Optimal strategies for determination of free/extractable and total microcystins in lake sediment[J]. ANALYTICA CHIMICA ACTA,2012,709(0):66-72. |
| APA | Wu, Xingqiang,Wang, Chunbo,Xiao, Bangding,Wang, Yang,Zheng, Na,&Liu, Jingshuang.(2012).Optimal strategies for determination of free/extractable and total microcystins in lake sediment.ANALYTICA CHIMICA ACTA,709(0),66-72. |
| MLA | Wu, Xingqiang,et al."Optimal strategies for determination of free/extractable and total microcystins in lake sediment".ANALYTICA CHIMICA ACTA 709.0(2012):66-72. |
入库方式： OAI收割
来源：水生生物研究所
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