Molecular cloning and characterization of interferon regulatory factor 1 (IRF-1), IRF-2 and IRF-5 in the chondrostean paddlefish Polyodon spathula and their phylogenetic importance in the Osteichthyes
文献类型：期刊论文
| 作者 | Gan Xiaoni1,2,3; Chen Zhuo2; Wang Xuzhen2; Wang Dengqiang2; Chen Xinwen1 |
| 刊名 | DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
 |
| 出版日期 | 2012 |
| 卷号 | 36期号:1页码:74-84 |
| 关键词 | IFN Innate immunity PsIRF-1 PsIRF-2 PsIRF-5 Polyodon spathula Phylogeny |
| ISSN号 | 0145-305X |
| 通讯作者 | Chen, XW (reprint author), Chinese Acad Sci, State Key Lab Virol, Wuhan Inst Virol, Wuhan 430071, Hubei Province, Peoples R China |
| 中文摘要 | The interferon regulatory factor (IRF) with its 10 members is a very important gene family related to innate immunity. Currently, most fish IRFs reported are from bony fish (teleosts). Cloning and sequencing of IRFs from chondrosteans, the so-called "ancient fish" including sturgeon, paddlefish, bichir and gar, are absent from the literature. In this study, three IRF genes PsIRF-1, PsIRF-2 and PsIRF-5, were cloned and characterized from the paddlefish (Polyodon spathula). PsIRF-1 includes an open reading frame (ORF) of 972 bp that encodes a putative protein of 324 amino acids; PsIRF-2 includes an ORF of 1023 bp encoding 341 amino acids and P5IRF-5 includes an ORF of 1491 bp that encodes 497 amino acids. The P5IRF-5 gene structure is similar to those in mammals but differs from those in teleosts in the first and second exons. Phylogenetic studies of the putative amino acid sequences of PsIRF-1, PsIRF-2 and PsIRF-5 based on the neighbor-joining and Bayesian inference method for Osteichthyes found widely accepted inter-relationships among actinopterygians and tetrapods. Reverse Transcription Polymerase Chain Reaction (RT-PCR) analysis of PsIRF-1, PsIRF-2 and P5IRF-5 in different paddlefish tissues shows higher levels of expression in gill, spleen and head kidney. Poly (I: C) (polyinosinic-polycytidylic acid) stimulation in vivo up-regulated PsIRF-1 and PsIRF-2 expression, while P5IRF-5 gene expression did not respond to the challenge of Poly (I: C). (C) 2011 Elsevier Ltd. All rights reserved. |
| 英文摘要 | The interferon regulatory factor (IRF) with its 10 members is a very important gene family related to innate immunity. Currently, most fish IRFs reported are from bony fish (teleosts). Cloning and sequencing of IRFs from chondrosteans, the so-called "ancient fish" including sturgeon, paddlefish, bichir and gar, are absent from the literature. In this study, three IRF genes PsIRF-1, PsIRF-2 and PsIRF-5, were cloned and characterized from the paddlefish (Polyodon spathula). PsIRF-1 includes an open reading frame (ORF) of 972 bp that encodes a putative protein of 324 amino acids; PsIRF-2 includes an ORF of 1023 bp encoding 341 amino acids and P5IRF-5 includes an ORF of 1491 bp that encodes 497 amino acids. The P5IRF-5 gene structure is similar to those in mammals but differs from those in teleosts in the first and second exons. Phylogenetic studies of the putative amino acid sequences of PsIRF-1, PsIRF-2 and PsIRF-5 based on the neighbor-joining and Bayesian inference method for Osteichthyes found widely accepted inter-relationships among actinopterygians and tetrapods. Reverse Transcription Polymerase Chain Reaction (RT-PCR) analysis of PsIRF-1, PsIRF-2 and P5IRF-5 in different paddlefish tissues shows higher levels of expression in gill, spleen and head kidney. Poly (I: C) (polyinosinic-polycytidylic acid) stimulation in vivo up-regulated PsIRF-1 and PsIRF-2 expression, while P5IRF-5 gene expression did not respond to the challenge of Poly (I: C). (C) 2011 Elsevier Ltd. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Immunology ; Zoology |
| 研究领域[WOS] | Immunology ; Zoology |
| 关键词[WOS] | GENE-EXPRESSION ; TRANSCRIPTION ; ACTIVATION ; PROMOTER ; GENERATION ; INDUCTION ; SEQUENCE ; CELLS ; FISH ; RNA |
| 收录类别 | SCI |
| 资助信息 | Natural Science Foundation of China[HCV2009CB522500, 30950006] |
| 语种 | 英语 |
| WOS记录号 | WOS:000299390300008 |
| 公开日期 | 2012-04-01 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/16703]  |
| 专题 | 水生生物研究所_水生生物多样性与资源保护研究中心_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, State Key Lab Virol, Wuhan Inst Virol, Wuhan 430071, Hubei Province, Peoples R China 2.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei Province, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Gan Xiaoni,Chen Zhuo,Wang Xuzhen,et al. Molecular cloning and characterization of interferon regulatory factor 1 (IRF-1), IRF-2 and IRF-5 in the chondrostean paddlefish Polyodon spathula and their phylogenetic importance in the Osteichthyes[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2012,36(1):74-84. |
| APA | Gan Xiaoni,Chen Zhuo,Wang Xuzhen,Wang Dengqiang,&Chen Xinwen.(2012).Molecular cloning and characterization of interferon regulatory factor 1 (IRF-1), IRF-2 and IRF-5 in the chondrostean paddlefish Polyodon spathula and their phylogenetic importance in the Osteichthyes.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,36(1),74-84. |
| MLA | Gan Xiaoni,et al."Molecular cloning and characterization of interferon regulatory factor 1 (IRF-1), IRF-2 and IRF-5 in the chondrostean paddlefish Polyodon spathula and their phylogenetic importance in the Osteichthyes".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 36.1(2012):74-84. |
入库方式： OAI收割
来源：水生生物研究所
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