The proteomic study on cellular responses of the testes of zebrafish (Danio rerio) exposed to microcystin-RR
文献类型：期刊论文
| 作者 | Zhao, Sujuan1; Xie, Ping1; Li, Guangyu1,2; Jun, Chen1; Cai, Yan1; Xiong, Qian1; Zhao, Yanyan1 |
| 刊名 | PROTEOMICS
 |
| 出版日期 | 2012 |
| 卷号 | 12期号:2页码:300-312 |
| 关键词 | 2-DE Animal proteomics MC-RR Reproductive toxicity Testis Ultrastructure |
| ISSN号 | 1615-9853 |
| 通讯作者 | Xie, P (reprint author), Chinese Acad Sci, Donghu Expt Stn Lake Ecosyst, State Key Lab Freshwater Ecol & Biotechnol China, Inst Hydrobiol, Donghu S Rd 7, Wuhan 430072, Peoples R China |
| 中文摘要 | Microcystin-RR (MC-RR) is a commonly encountered cyanotoxin and receives increasing attention due to the risk of its bioaccumulation in aquatic animals like fish. This study investigated the protein profiles of zebrafish (Danio rerio) testes after intraperitoneal injection (i.p.) with 0.5 LD50 (2000 mu g/kg). MC-RR caused a noticeable damage to testicular ultrastructure, showing widened intercellular junction, distention of mitochondria. The testes showed a rapid response of its defense systems to the oxidative stress caused by MC-RR. This is the first to use a proteomic approach to obtain an overview of the effects of MC-RR on the testes of zebrafish. The proteomic results revealed that toxin exposure remarkably altered the abundance of 24 proteins that were involved in cytoskeleton assembly, oxidative stress, glycolysis metabolism, calcium ion binding and other biological functions. In conclusion, MC-RR damaged the testes and was toxic to the reproductive system of male zebrafish mainly through causing oxidative stress. |
| 英文摘要 | Microcystin-RR (MC-RR) is a commonly encountered cyanotoxin and receives increasing attention due to the risk of its bioaccumulation in aquatic animals like fish. This study investigated the protein profiles of zebrafish (Danio rerio) testes after intraperitoneal injection (i.p.) with 0.5 LD50 (2000 mu g/kg). MC-RR caused a noticeable damage to testicular ultrastructure, showing widened intercellular junction, distention of mitochondria. The testes showed a rapid response of its defense systems to the oxidative stress caused by MC-RR. This is the first to use a proteomic approach to obtain an overview of the effects of MC-RR on the testes of zebrafish. The proteomic results revealed that toxin exposure remarkably altered the abundance of 24 proteins that were involved in cytoskeleton assembly, oxidative stress, glycolysis metabolism, calcium ion binding and other biological functions. In conclusion, MC-RR damaged the testes and was toxic to the reproductive system of male zebrafish mainly through causing oxidative stress. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biochemical Research Methods ; Biochemistry & Molecular Biology |
| 研究领域[WOS] | Biochemistry & Molecular Biology |
| 关键词[WOS] | CARP CYPRINUS-CARPIO ; ALDEHYDE DEHYDROGENASE ; INTERMEDIATE-FILAMENTS ; REPRODUCTIVE-SYSTEM ; OXIDATIVE STRESS ; HUMAN HEALTH ; LR ; LIVER ; GLUTATHIONE ; TOXICITY |
| 收录类别 | SCI |
| 资助信息 | National Basic Research Program of China (973 Program)[2008CB418101]; China's Ministry of Environmental Protection[2008ZX07101-001]; State Key Laboratory of Freshwater Ecology and Biotechnology[2008FBZ01] |
| 语种 | 英语 |
| WOS记录号 | WOS:000299153500014 |
| 公开日期 | 2012-04-01 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/16717]  |
| 专题 | 水生生物研究所_淡水生态学研究中心_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Donghu Expt Stn Lake Ecosyst, State Key Lab Freshwater Ecol & Biotechnol China, Inst Hydrobiol, Wuhan 430072, Peoples R China 2.Huazhong Agr Univ, Coll Fisheries, Wuhan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, Sujuan,Xie, Ping,Li, Guangyu,et al. The proteomic study on cellular responses of the testes of zebrafish (Danio rerio) exposed to microcystin-RR[J]. PROTEOMICS,2012,12(2):300-312. |
| APA | Zhao, Sujuan.,Xie, Ping.,Li, Guangyu.,Jun, Chen.,Cai, Yan.,...&Zhao, Yanyan.(2012).The proteomic study on cellular responses of the testes of zebrafish (Danio rerio) exposed to microcystin-RR.PROTEOMICS,12(2),300-312. |
| MLA | Zhao, Sujuan,et al."The proteomic study on cellular responses of the testes of zebrafish (Danio rerio) exposed to microcystin-RR".PROTEOMICS 12.2(2012):300-312. |
入库方式： OAI收割
来源：水生生物研究所
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