The role of cysteine conjugation in the detoxification of microcystin-LR in liver of bighead carp (Aristichthys nobilis): a field and laboratory study
文献类型：期刊论文
| 作者 | Zhang, Dawen1,2; Yang, Qiang1; Xie, Ping1; Deng, Xuwei1; Chen, Jun1; Dai, Ming1 |
| 刊名 | ECOTOXICOLOGY
 |
| 出版日期 | 2012 |
| 卷号 | 21期号:1页码:244-252 |
| 关键词 | Microcystins MC-LR-Cys MC-LR-GSH Bighead carp Detoxification |
| ISSN号 | 0963-9292 |
| 通讯作者 | Chen, J (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol China, Donghu Expt Stn Lake Ecosyst, Donghu S Rd 7, Wuhan 430072, Peoples R China |
| 中文摘要 | The role of glutathione (GSH) and cysteine (Cys) conjugates in the detoxification of microcystin-LR (MC-LR) in bighead carp (Aristichthys nobilis) was examined under laboratory and field conditions. Wild individuals of bighead carp were collected from 5 eutrophic lakes along the Yangtze River, while in laboratory experiment, bighead carp were injected intraperitoneally with 500 mu g purified MC-LR/kg body weight (bw). Contents of MC-LR and its glutathione (MC-LR-GSH) and cysteine conjugates (MC-LR-Cys) in the liver of bighead carp were determined by liquid chromatography electrospray ionization mass spectrum (LC-ESI-MS). In laboratory experiment, low concentrations of MC-LR-GSH (mean: 0.042 mu g/g dry weight (DW)) were always detectable, and the mean ratio of MC-LR-Cys to MC-LR-GSH was 6.55. While, in field study, relatively high MC-LR-Cys concentration (mean: 0.22 mu g/g DW) was detected, whereas MC-LR-GSH was occasionally detectable, and the average ratio of MC-LR-Cys to MC-LR-GSH was as high as 71.49. A positive correlation was found between MC-LR-Cys concentration in the liver of bighead carp and MC-LR content in seston from the five lakes (r = 0.85). These results suggest that MC-LR-Cys might be much more important than MC-LR-GSH in the detoxification of MC-LR in fish liver, and that cysteine conjugation of MC-LR might be a physiological mechanism for the phytoplanktivorous bighead carp to counteract toxic cyanobacteria. |
| 英文摘要 | The role of glutathione (GSH) and cysteine (Cys) conjugates in the detoxification of microcystin-LR (MC-LR) in bighead carp (Aristichthys nobilis) was examined under laboratory and field conditions. Wild individuals of bighead carp were collected from 5 eutrophic lakes along the Yangtze River, while in laboratory experiment, bighead carp were injected intraperitoneally with 500 mu g purified MC-LR/kg body weight (bw). Contents of MC-LR and its glutathione (MC-LR-GSH) and cysteine conjugates (MC-LR-Cys) in the liver of bighead carp were determined by liquid chromatography electrospray ionization mass spectrum (LC-ESI-MS). In laboratory experiment, low concentrations of MC-LR-GSH (mean: 0.042 mu g/g dry weight (DW)) were always detectable, and the mean ratio of MC-LR-Cys to MC-LR-GSH was 6.55. While, in field study, relatively high MC-LR-Cys concentration (mean: 0.22 mu g/g DW) was detected, whereas MC-LR-GSH was occasionally detectable, and the average ratio of MC-LR-Cys to MC-LR-GSH was as high as 71.49. A positive correlation was found between MC-LR-Cys concentration in the liver of bighead carp and MC-LR content in seston from the five lakes (r = 0.85). These results suggest that MC-LR-Cys might be much more important than MC-LR-GSH in the detoxification of MC-LR in fish liver, and that cysteine conjugation of MC-LR might be a physiological mechanism for the phytoplanktivorous bighead carp to counteract toxic cyanobacteria. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Ecology ; Environmental Sciences ; Toxicology |
| 研究领域[WOS] | Environmental Sciences & Ecology ; Toxicology |
| 关键词[WOS] | HYPOPHTHALMICHTHYS-MOLITRIX ; HEPATOTOXIC MICROCYSTINS ; CYANOBACTERIAL TOXINS ; GLUTATHIONE CONJUGATE ; IN-VIVO ; IDENTIFICATION ; TOXICITY ; BRAZIL ; FISH ; LAKE |
| 收录类别 | SCI |
| 资助信息 | Ministry of Science and Technology of China[2006FY110600]; State Key Laboratory of Freshwater Ecology and Biotechnology[2008FBZ01] |
| 语种 | 英语 |
| WOS记录号 | WOS:000298996900025 |
| 公开日期 | 2012-04-01 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/16723]  |
| 专题 | 水生生物研究所_淡水生态学研究中心_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol China, Donghu Expt Stn Lake Ecosyst, Wuhan 430072, Peoples R China 2.Jiangxi Acad Agr Sci, Inst Qual & Safety & Stand Agr Prod Res, Key Lab Qual & Safety Agr Prod, Nanchang 330200, Jiangxi, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Dawen,Yang, Qiang,Xie, Ping,et al. The role of cysteine conjugation in the detoxification of microcystin-LR in liver of bighead carp (Aristichthys nobilis): a field and laboratory study[J]. ECOTOXICOLOGY,2012,21(1):244-252. |
| APA | Zhang, Dawen,Yang, Qiang,Xie, Ping,Deng, Xuwei,Chen, Jun,&Dai, Ming.(2012).The role of cysteine conjugation in the detoxification of microcystin-LR in liver of bighead carp (Aristichthys nobilis): a field and laboratory study.ECOTOXICOLOGY,21(1),244-252. |
| MLA | Zhang, Dawen,et al."The role of cysteine conjugation in the detoxification of microcystin-LR in liver of bighead carp (Aristichthys nobilis): a field and laboratory study".ECOTOXICOLOGY 21.1(2012):244-252. |
入库方式： OAI收割
来源：水生生物研究所
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