Transcription of Il17 and Il17f Is Controlled by Conserved Noncoding Sequence 2
文献类型：期刊论文
| 作者 | Wang, Xiaohu1; Zhang, Yibing1,2; Yang, Xuexian O.1; Nurieva, Roza I.1; Chang, Seon Hee1; Ojeda, Sandra S.1; Kang, Hong S.3; Schluns, Kimberly S.1; Gui, Jianfang2; Jetten, Anton M.3 |
| 刊名 | IMMUNITY
 |
| 出版日期 | 2012-01-27 |
| 卷号 | 36期号:1页码:23-31 |
| ISSN号 | 1074-7613 |
| 通讯作者 | Dong, C (reprint author), Univ Texas MD Anderson Canc Ctr, Dept Immunol, Houston, TX 77030 USA |
| 中文摘要 | T helper 17 (Th17) cells specifically transcribe the Il17 and Il17f genes, which are localized in the same chromosome region, but the underlying mechanism is unclear. Here, we report a cis element that we previously named conserved noncoding sequence 2 (CNS2) physically interacted with both Il17 and Il17f gene promoters and was sufficient for regulating their selective transcription in Th17 cells. Targeted deletion of CNS2 resulted in impaired retinoic acid-related orphan receptor gammat (ROR gamma t)-driven IL-17 expression in vitro. CNS2-deficient T cells also produced substantially decreased amounts of IL-17F. These cytokine defects were associated with defective chromatin remodeling in the Ild17-Il17f gene locus, possibly because of effects on CNS2-mediated recruitment of histone-modifying enzymes p300 and JmjC domain-containing protein 3 (JMJD3). CNS2-deficient animals were also shown to be resistant to experimental autoimmune encephalomyelitis (EAE). Our results thus suggest that CNS2 is sufficient and necessary for Il17 and optimal Il17f gene transcription in Th17 cells. |
| 英文摘要 | T helper 17 (Th17) cells specifically transcribe the Il17 and Il17f genes, which are localized in the same chromosome region, but the underlying mechanism is unclear. Here, we report a cis element that we previously named conserved noncoding sequence 2 (CNS2) physically interacted with both Il17 and Il17f gene promoters and was sufficient for regulating their selective transcription in Th17 cells. Targeted deletion of CNS2 resulted in impaired retinoic acid-related orphan receptor gammat (ROR gamma t)-driven IL-17 expression in vitro. CNS2-deficient T cells also produced substantially decreased amounts of IL-17F. These cytokine defects were associated with defective chromatin remodeling in the Ild17-Il17f gene locus, possibly because of effects on CNS2-mediated recruitment of histone-modifying enzymes p300 and JmjC domain-containing protein 3 (JMJD3). CNS2-deficient animals were also shown to be resistant to experimental autoimmune encephalomyelitis (EAE). Our results thus suggest that CNS2 is sufficient and necessary for Il17 and optimal Il17f gene transcription in Th17 cells. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Immunology |
| 研究领域[WOS] | Immunology |
| 关键词[WOS] | EXPERIMENTAL AUTOIMMUNE ENCEPHALOMYELITIS ; RANGE INTRACHROMOSOMAL INTERACTIONS ; CD4(+) T-CELLS ; ROR-GAMMA-T ; NUCLEAR RECEPTORS ; GENE-EXPRESSION ; DIFFERENTIATION ; CYTOKINE ; LOCUS ; LINEAGE |
| 收录类别 | SCI |
| 资助信息 | NIH; American Heart Association; 973 National Basic Research Program of China[2010CB126301]; National Multiple Sclerosis Society; Chinese Academy of Sciences; MD Anderson Cancer Center; Leukemia and Lymphoma Society |
| 语种 | 英语 |
| WOS记录号 | WOS:000299766000007 |
| 公开日期 | 2012-04-01 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/16731]  |
| 专题 | 水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文 |
| 作者单位 | 1.Univ Texas MD Anderson Canc Ctr, Dept Immunol, Houston, TX 77030 USA 2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China 3.NIEHS, Cell Biol Sect, LRB, NIH, Res Triangle Pk, NC 27709 USA |
| 推荐引用方式 GB/T 7714 | Wang, Xiaohu,Zhang, Yibing,Yang, Xuexian O.,et al. Transcription of Il17 and Il17f Is Controlled by Conserved Noncoding Sequence 2[J]. IMMUNITY,2012,36(1):23-31. |
| APA | Wang, Xiaohu.,Zhang, Yibing.,Yang, Xuexian O..,Nurieva, Roza I..,Chang, Seon Hee.,...&Dong, Chen.(2012).Transcription of Il17 and Il17f Is Controlled by Conserved Noncoding Sequence 2.IMMUNITY,36(1),23-31. |
| MLA | Wang, Xiaohu,et al."Transcription of Il17 and Il17f Is Controlled by Conserved Noncoding Sequence 2".IMMUNITY 36.1(2012):23-31. |
入库方式： OAI收割
来源：水生生物研究所
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