Construction of two selectable markers for integrative/conjugative plasmids in Flavobacterium columnare
文献类型：期刊论文
| 作者 | Zhang Jin1,2; Zou Hong1; Wang Liangfa1; Huang Bei1; Li Nan1; Wang Guitang1; Nie Pin1 |
| 刊名 | CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
 |
| 出版日期 | 2012-03-01 |
| 卷号 | 30期号:2页码:269-278 |
| 关键词 | Flavobacterium columnare selectable marker integrative/conjugative plasmid gene disruption |
| ISSN号 | 0254-4059 |
| 通讯作者 | Nie, P (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
| 中文摘要 | Flavobacterium columnare, the etiological agent of columnaris disease, is one of the most important and widespread bacterial pathogens of freshwater fish. In this study, we constructed two artificial selectable markers (chloramphenicol and spectinomycin resistance) for gene transfer in F. columnare. These two new artificial selectable markers, which were created by placing the chloramphenicol or spectinomycin resistance gene under the control of the native acs regulatory region of F. columnare, were functional in both F. columnare and Escherichia coli. The integrative/conjugative plasmids constructed by using these markers were introduced into F. columnare G(4) via electroporation or conjugation. The integrated plasmid DNA was confirmed by Southern blotting and PCR analysis. These two markers can be employed in future investigations into gene deletion and the pathogenicity of virulence factors in F. columnare. |
| 英文摘要 | Flavobacterium columnare, the etiological agent of columnaris disease, is one of the most important and widespread bacterial pathogens of freshwater fish. In this study, we constructed two artificial selectable markers (chloramphenicol and spectinomycin resistance) for gene transfer in F. columnare. These two new artificial selectable markers, which were created by placing the chloramphenicol or spectinomycin resistance gene under the control of the native acs regulatory region of F. columnare, were functional in both F. columnare and Escherichia coli. The integrative/conjugative plasmids constructed by using these markers were introduced into F. columnare G(4) via electroporation or conjugation. The integrated plasmid DNA was confirmed by Southern blotting and PCR analysis. These two markers can be employed in future investigations into gene deletion and the pathogenicity of virulence factors in F. columnare. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
| 类目[WOS] | Limnology ; Oceanography |
| 研究领域[WOS] | Marine & Freshwater Biology ; Oceanography |
| 关键词[WOS] | CHONDROITIN AC LYASE ; CATFISH ICTALURUS-PUNCTATUS ; GLIDING MOTILITY ; CHANNEL CATFISH ; FLEXIBACTER-COLUMNARIS ; GENETIC MANIPULATION ; BACTEROIDES-FRAGILIS ; CYTOPHAGA JOHNSONAE ; ESCHERICHIA-COLI ; LOW VIRULENCE |
| 收录类别 | SCI |
| 资助信息 | Chinese Ministry of Science and Technology[2011CB944600]; US Department of Energy[DE-FG02-02ER15332]; Fudan University; Rijk Zwaan; Institute of Hydrobiology; Biology Department; Huck Institutes of the Life Sciences at the Pennsylvania State University |
| 语种 | 英语 |
| WOS记录号 | WOS:000301001900011 |
| 公开日期 | 2012-04-01 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/16741]  |
| 专题 | 水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang Jin,Zou Hong,Wang Liangfa,et al. Construction of two selectable markers for integrative/conjugative plasmids in Flavobacterium columnare[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2012,30(2):269-278. |
| APA | Zhang Jin.,Zou Hong.,Wang Liangfa.,Huang Bei.,Li Nan.,...&Nie Pin.(2012).Construction of two selectable markers for integrative/conjugative plasmids in Flavobacterium columnare.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,30(2),269-278. |
| MLA | Zhang Jin,et al."Construction of two selectable markers for integrative/conjugative plasmids in Flavobacterium columnare".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 30.2(2012):269-278. |
入库方式： OAI收割
来源：水生生物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。