Bioreducible crosslinked low molecular weight branched PEI-PBLG as an efficient gene carrier
文献类型：期刊论文
| 作者 | Guo ZP ; Tian HY ; Xia JL ; Chen J ; Lin L ; Chen XS |
| 刊名 | science china-chemistry
 |
| 出版日期 | 2010 |
| 卷号 | 53期号:12页码:2490-2496 |
| 关键词 | IN-VIVO TRANSFECTION EFFICIENCY DELIVERY-SYSTEMS DNA POLYETHYLENIMINE GLUTATHIONE VECTOR CELLS COPOLYMERS POLYMERS |
| ISSN号 | 1674-7291 |
| 通讯作者 | chen xs |
| 中文摘要 | low molecular weight polyethylenimine-poly(gamma-benzyl l-glutamate) (pei-pblg) was crosslinked by n,n'-cystamine-bisacrylamide (cba) to get the polymer named as cba-pei-pblg (cpp). cpp not only inherits pei-pblg's amphiphilic advantages, but also possesses reducible properties. cpp can complex with dna to form nanoparticles. cpp/dna complex particles were characterized by particle size and zeta potential analysis. the result showed that the complex particles have suitable size and surface charges for gene delivery. and gel retardation assay also prove cba-pei-pblg has proper condensing ability for dna. cpp has good reducible property, and also has good biocompatibility because of introducing pblg segment. the cytotoxicity of cpp was evaluated using mtt assay, and the results showed cpp has lower cytotoxicity compared with pei 25 k. the transfection properties were characterized in different cells by using plasmid dna as a reporter. cpp showed higher transfection efficiencies and lower cytotoxicity in hela cells. this was attributed to bioreducible and biocompatibility properties of the cpp. these results suggested that cpp is a promising low-toxic, highly effective non-viral gene carrier. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000285366200009 |
| 公开日期 | 2012-04-25 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/43239]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Guo ZP,Tian HY,Xia JL,et al. Bioreducible crosslinked low molecular weight branched PEI-PBLG as an efficient gene carrier[J]. science china-chemistry,2010,53(12):2490-2496. |
| APA | Guo ZP,Tian HY,Xia JL,Chen J,Lin L,&Chen XS.(2010).Bioreducible crosslinked low molecular weight branched PEI-PBLG as an efficient gene carrier.science china-chemistry,53(12),2490-2496. |
| MLA | Guo ZP,et al."Bioreducible crosslinked low molecular weight branched PEI-PBLG as an efficient gene carrier".science china-chemistry 53.12(2010):2490-2496. |
入库方式： OAI收割
来源：长春应用化学研究所
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