Viable but nonculturable cells used in biosensor fabrication for long-term storage stability
文献类型：期刊论文
| 作者 | Liu L ; Liu CY ; Shang L ; Li D ; Yong DM ; Qi L ; Dong SJ |
| 刊名 | talanta
 |
| 出版日期 | 2010 |
| 卷号 | 83期号:1页码:31-35 |
| 关键词 | ENTEROCOCCUS-FAECALIS VIBRIO-PARAHAEMOLYTICUS LIVING CELLS MORPHOLOGY 4-VINYLPYRIDINE RESUSCITATION NANOWIRES SURVIVAL OXYGEN ASSAYS |
| ISSN号 | 0039-9140 |
| 通讯作者 | dong sj |
| 中文摘要 | in this paper we first reported the viable but nonculturable (vbnc) cells used for fabricating biosensor the organic-inorganic hybrid material composed of silica and the grafting copolymer of poly(vinyl alcohol) and 4-vinylpyridine (pva-g-p(4-vp)) was used to immobilize microbial cells for biosensor fabrication the vbnc cells were formed after the hybrid material dried showing the cell walls were sacrificed with the intracellular enzymes as core and the sacrificed" cell walls as shell the present vbnc cells maybe considered as a core/shell structure the extracellular material worked as the scaffold for core/shell structure the core/shell structure and the scaffold structure were demonstrated by single-cell level image analysis using confocal laser scanning microscopy (clsm) the electrochemical method was adopted for further examining the enzyme activity of vbnc cells the vbnc cells did not need nutrient treatment and other physicochemical factors for cell growth which is a significant contribution for storing biosensor a glucose-glutamic acid biosensor fabricated by the vbnc cells exhibited long-term storage stability for 100 days (c) 2010 elsevier b v all rights reserved |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000284972800005 |
| 公开日期 | 2012-04-25 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/43250]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Liu L,Liu CY,Shang L,et al. Viable but nonculturable cells used in biosensor fabrication for long-term storage stability[J]. talanta,2010,83(1):31-35. |
| APA | Liu L.,Liu CY.,Shang L.,Li D.,Yong DM.,...&Dong SJ.(2010).Viable but nonculturable cells used in biosensor fabrication for long-term storage stability.talanta,83(1),31-35. |
| MLA | Liu L,et al."Viable but nonculturable cells used in biosensor fabrication for long-term storage stability".talanta 83.1(2010):31-35. |
入库方式： OAI收割
来源：长春应用化学研究所
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