DNA/Ligand/lon-Based Ensemble for Fluorescence Turn on Detection of Cysteine and Histidine with Tunable Dynamic Range
文献类型：期刊论文
| 作者 | Pu F ; Huang ZZ ; Ren JS ; Qu XG |
| 刊名 | analytical chemistry
 |
| 出版日期 | 2010 |
| 卷号 | 82期号:19页码:8211-8216 |
| 关键词 | PERFORMANCE LIQUID-CHROMATOGRAPHY CAPILLARY-ZONE-ELECTROPHORESIS INTERCALATED ETHIDIUM-BROMIDE ALPHA-AMINO-ACIDS ELECTROCHEMICAL DETECTION SPECTROFLUOROMETRIC DETERMINATION PRECOLUMN DERIVATIZATION SELECTIVE DETECTION GOLD NANOPARTICLES METAL-IONS |
| ISSN号 | 0003-2700 |
| 通讯作者 | ren js |
| 中文摘要 | a new type of rapid, highly sensitive, and selective fluorescence turn-on assay for detection of cysteine and histidine using a dna/ligand/ion ensemble is developed. this assay is based on the highly specific interaction between the amino acids and the metal ions and the strong fluorescence thiazole orange (to)/dna probe in a competition assay format. the resulting high sensitivity and selectivity for cysteine and histidine was achieved by changing the metal ions. the system is simple in design and fast in operation and is more convenient and promising than other methods. the novel strategy eliminated the need of organic cosolvents, enzymatic reactions, separation processes, chemical modifications, and sophisticated instrumentations. the detection and discrimination process can be seen with the naked eye under a hand-held uv lamp and can be easily adapted to automated high-throughput screening. the detection limit of this method is lower than or at least comparable to previous fluorescence-based methods. the dynamic range of the sensor can be tuned simply by adjusting the concentration of metal ions. importantly, the protocol offers high selectivity for the determination of cysteine among amino acids found in proteins and in serum samples. the assay shows great potential for practical application as a disease-associated biomarker and will be needed to satisfy the great demand of amino acid determination in fields such as food processing, biochemistry, pharmaceuticals, and clinical analysis. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000282257400047 |
| 公开日期 | 2012-04-25 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/43365]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Pu F,Huang ZZ,Ren JS,et al. DNA/Ligand/lon-Based Ensemble for Fluorescence Turn on Detection of Cysteine and Histidine with Tunable Dynamic Range[J]. analytical chemistry,2010,82(19):8211-8216. |
| APA | Pu F,Huang ZZ,Ren JS,&Qu XG.(2010).DNA/Ligand/lon-Based Ensemble for Fluorescence Turn on Detection of Cysteine and Histidine with Tunable Dynamic Range.analytical chemistry,82(19),8211-8216. |
| MLA | Pu F,et al."DNA/Ligand/lon-Based Ensemble for Fluorescence Turn on Detection of Cysteine and Histidine with Tunable Dynamic Range".analytical chemistry 82.19(2010):8211-8216. |
入库方式： OAI收割
来源：长春应用化学研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。