Isolation and characterization of cytotoxic cyclotides from Viola philippica
文献类型：期刊论文
| 作者 | He, Wenjun1,3; Chan, Lai Yue2; Zeng, Guangzhi1; Daly, Norelle L.2; Craik, David J.2; Tan, Ninghua1 |
| 刊名 | PEPTIDES
 |
| 出版日期 | 2011-08-01 |
| 卷号 | 32期号:8页码:1719-1723 |
| 关键词 | Cyclotides Cyclic peptides Violaceae Viola philippica Cytotoxic activity Viphi A-H |
| ISSN号 | 0196-9781 |
| 通讯作者 | Tan, NH (reprint author), Chinese Acad Sci, State Key Lab Phytochem & Plant Resources W China, Kunming Inst Bot, 132 Lanhei Rd, Kunming 650204, Yunnan, Peoples R China |
| 英文摘要 | Cyclotides are a large family of plant peptides characterized by a macrocyclic backbone and knotted arrangement of three disulfide bonds. This unique structure renders cyclotides exceptionally stable to thermal, chemical and enzymatic treatments. They exhibit a variety of bioactivities, including uterotonic, anti-HIV, cytotoxic and hemolytic activity and it is these properties that make cyclotides an interesting peptide scaffold for drug design. In this study, eight new cyclotides (Viphi A-H), along with eight known cyclotides, were isolated from Viola philippica, a plant from the Violaceae family. In addition, Viba 17 and Mram 8 were isolated for the first time as peptides. The sequences of these cyclotides were elucidated primarily by using a strategy involving reduction, enzymatic digestion and tandem mass spectroscopy sequencing. Several of the cyclotides showed cytotoxic activities against the cancer cell lines MM96L, HeLa and BGC-823. The novel cyclotides reported here: (1) enhance the known sequence variation observed for cyclotides; (2) extend the number of species known to contain cyclotides; (3) provide interesting structure-activity relationships that delineate residues important for cytotoxic activity. In addition, this study provides insights into the potential active ingredients of traditional Chinese medicines. (C) 2011 Elsevier Inc. All rights reserved. |
| 学科主题 | Biochemistry & Molecular Biology; Pharmacology & Pharmacy |
| 类目[WOS] | Biochemistry & Molecular Biology ; Pharmacology & Pharmacy |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Pharmacology & Pharmacy |
| 关键词[WOS] | CYCLIC CYSTINE KNOT ; CYCLOVIOLACIN O2 ; OLDENLANDIA-AFFINIS ; CIRCULAR PROTEINS ; MACROCYCLIC PEPTIDES ; SEQUENCE VARIATION ; STRUCTURAL MOTIF ; PLANT CYCLOTIDES ; KALATA B1 ; BIOSYNTHESIS |
| 收录类别 | SCI |
| 资助信息 | NSFC, ARC; National Natural Science Foundation of China[30725048, 91013002, U1032602]; National New Drug Innovation Great Project of China[2011ZX09307-002-02]; National Basic Research Program of China[2009C8522300]; China Scholarship Council |
| 语种 | 英语 |
| WOS记录号 | WOS:000294572800021 |
| 公开日期 | 2012-04-10 |
| 源URL | [http://ir.kib.ac.cn/handle/151853/5357]  |
| 专题 | 昆明植物研究所_植物化学与西部植物资源持续利用国家重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, State Key Lab Phytochem & Plant Resources W China, Kunming Inst Bot, Kunming 650204, Yunnan, Peoples R China 2.Univ Queensland, Inst Mol Biosci, Brisbane, Qld 4072, Australia 3.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | He, Wenjun,Chan, Lai Yue,Zeng, Guangzhi,et al. Isolation and characterization of cytotoxic cyclotides from Viola philippica[J]. PEPTIDES,2011,32(8):1719-1723. |
| APA | He, Wenjun,Chan, Lai Yue,Zeng, Guangzhi,Daly, Norelle L.,Craik, David J.,&Tan, Ninghua.(2011).Isolation and characterization of cytotoxic cyclotides from Viola philippica.PEPTIDES,32(8),1719-1723. |
| MLA | He, Wenjun,et al."Isolation and characterization of cytotoxic cyclotides from Viola philippica".PEPTIDES 32.8(2011):1719-1723. |
入库方式： OAI收割
来源：昆明植物研究所
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