Analysis of secretome of breast cancer cell line with an optimized semi-shotgun method
文献类型：期刊论文
| 作者 | Tang, XR ; Yao, L ; Chen, KY ; Hu, XF ; Xu, LX ; Fan, CH(樊春海） |
| 刊名 | NUCLEAR SCIENCE AND TECHNIQUES
 |
| 出版日期 | 2009 |
| 卷号 | 20期号:5页码:6 |
| 关键词 | Secretome Semi-shotgun Breast cancer |
| ISSN号 | 1001-8042 |
| 通讯作者 | Xu, LX (reprint author), Shanghai Jiao Tong Univ, Key Lab Syst Biomed, China Minist Educ, Shanghai 200240, Peoples R China |
| 英文摘要 | Secretome, the totality of secreted proteins, is viewed as a promising pool of candidate cancer biomarkers. Simple and reliable methods for identifying secreted proteins are highly desired. We used an optimized semi-shotgun liquid chromatography followed by tandem mass spectrometry (LC-MS/MS) method to analyze the secretome of breast cancer cell line MDA-MB-231. A total of 464 proteins were identified. About 63% of the proteins were classified as secreted proteins, including many promising breast cancer biomarkers, which were thought to be correlated with tumorigenesis, tumor development and metastasis. These results suggest that the optimized method may be a powerful strategy for cell line secretome profiling, and can be used to find potential cancer biomarkers with great clinical significance. |
| 学科主题 | Nuclear Science & Technology; Physics |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000273376100007 |
| 公开日期 | 2012-04-11 |
| 源URL | [http://ir.sinap.ac.cn/handle/331007/6836]  |
| 专题 | 上海应用物理研究所_中科院上海应用物理研究所2004-2010年 |
| 推荐引用方式 GB/T 7714 | Tang, XR,Yao, L,Chen, KY,et al. Analysis of secretome of breast cancer cell line with an optimized semi-shotgun method[J]. NUCLEAR SCIENCE AND TECHNIQUES,2009,20(5):6. |
| APA | Tang, XR,Yao, L,Chen, KY,Hu, XF,Xu, LX,&Fan, CH.(2009).Analysis of secretome of breast cancer cell line with an optimized semi-shotgun method.NUCLEAR SCIENCE AND TECHNIQUES,20(5),6. |
| MLA | Tang, XR,et al."Analysis of secretome of breast cancer cell line with an optimized semi-shotgun method".NUCLEAR SCIENCE AND TECHNIQUES 20.5(2009):6. |
入库方式： OAI收割
来源：上海应用物理研究所
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