Aqueous immune magnetite nanoparticles for immunoassay
文献类型：期刊论文
| 作者 | Zhang, GX(张国欣） ; Liu, YB ; Zhang, CF（张春富） ; Hu, WQ ; Xu, WB ; Li, Z ; Liang, S ; Cao, JQ（曹金全） ; Wang, YX（汪勇先） |
| 刊名 | JOURNAL OF NANOPARTICLE RESEARCH
 |
| 出版日期 | 2009 |
| 卷号 | 11期号:2页码:8 |
| 关键词 | Magnetite nanoparticles Silica-coating Surface modification Immunity Nanomedicine |
| ISSN号 | 1388-0764 |
| 通讯作者 | Wang, YX (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China |
| 英文摘要 | Immune magnetite nanoparticles (MNPs) are prepared by four successive reactions, which are MNPs preparation, silica-coating, surface modification with amino group, and conjugation with bio-molecule, respectively. The crystal structure and morphology of intermediate products are characterized by XRD, TEM and AFM. Qualitative and quantitative assays for amino group on the MNPs' surface are made by FTIR and Organic Element Assay. Ultraviolet-visible absorption spectrum can indirectly illustrate the quantity of bio-molecule conjugated with MNPs. In addition, specific combination and nonspecific combination of immune MNPs are measured by commercial RIA box. The results show that the size of MNPs prepared is 10 +/- A 5 nm, and silica-coated MNPs with spinel structure have quasi-spherical morphology. Infrared absorption bands of -NH(2) are appeared around 3380-3200 cm(-1) and 1650-1510 cm(-1), and the amino group content is 0.5 mu mol -NH(2) per mg MNPs. The specific immune combination of immune MNPs is up to 75%, and nonspecific combination is under 5%. |
| 学科主题 | Chemistry; Science & Technology - Other Topics; Materials Science |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000262987800018 |
| 公开日期 | 2012-04-11 |
| 源URL | [http://ir.sinap.ac.cn/handle/331007/6842]  |
| 专题 | 上海应用物理研究所_中科院上海应用物理研究所2004-2010年 |
| 推荐引用方式 GB/T 7714 | Zhang, GX,Liu, YB,Zhang, CF,et al. Aqueous immune magnetite nanoparticles for immunoassay[J]. JOURNAL OF NANOPARTICLE RESEARCH,2009,11(2):8. |
| APA | Zhang, GX.,Liu, YB.,Zhang, CF.,Hu, WQ.,Xu, WB.,...&Wang, YX.(2009).Aqueous immune magnetite nanoparticles for immunoassay.JOURNAL OF NANOPARTICLE RESEARCH,11(2),8. |
| MLA | Zhang, GX,et al."Aqueous immune magnetite nanoparticles for immunoassay".JOURNAL OF NANOPARTICLE RESEARCH 11.2(2009):8. |
入库方式： OAI收割
来源：上海应用物理研究所
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