DNA Structural Changes Under Different Stretching Methods Studied By Molecular Dynamics Simulations
文献类型：期刊论文
| 作者 | Qi, WP ; Lei, XL ; Fang, HP |
| 刊名 | CHEMPHYSCHEM
 |
| 出版日期 | 2010 |
| 卷号 | 11期号:10页码:6 |
| 关键词 | conformational analysis DNA genetic engineering molecular biology molecular dynamics |
| ISSN号 | 1439-4235 |
| 通讯作者 | Lei, XL (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, POB 800-204, Shanghai 201800, Peoples R China |
| 英文摘要 | We present a molecular dynamics simulation study of 22-mer DNA conformational variations obtained by stretching both 3'-termini and both 5'-termini. Stretching 3'-termini by 3.5 nm required 142 kJ mol(-1) and the force plateau was similar to 80 pN, whereas stretching 5'-termini by the same length required 190 kJ mol(-1) and the force plateau was similar to 100 pN. Stretching 3'-termini led to a larger untwisting of the double helix and the successive base pairs rolled to the side of the DNA minor groove, while stretching 5'-termini resulted in the base pairs rolling to the major groove side and reducing of the diameter of DNA molecule. The most distinctive difference between stretching 3'-termini and 5'-termini was that at the force plateau region stretching the 5'-termini resulted in breakage of the base pairs, which considerably disturbed the structure of the DNA double helix. All of the variations of base rotation and translation for both stretching methods took place when the relative length of DNA I was longer than 1.2, which was the point the force plateau appeared. |
| 学科主题 | Chemistry; Physics |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000280428400017 |
| 公开日期 | 2012-04-11 |
| 源URL | [http://ir.sinap.ac.cn/handle/331007/6890]  |
| 专题 | 上海应用物理研究所_中科院上海应用物理研究所2004-2010年 |
| 推荐引用方式 GB/T 7714 | Qi, WP,Lei, XL,Fang, HP. DNA Structural Changes Under Different Stretching Methods Studied By Molecular Dynamics Simulations[J]. CHEMPHYSCHEM,2010,11(10):6. |
| APA | Qi, WP,Lei, XL,&Fang, HP.(2010).DNA Structural Changes Under Different Stretching Methods Studied By Molecular Dynamics Simulations.CHEMPHYSCHEM,11(10),6. |
| MLA | Qi, WP,et al."DNA Structural Changes Under Different Stretching Methods Studied By Molecular Dynamics Simulations".CHEMPHYSCHEM 11.10(2010):6. |
入库方式： OAI收割
来源：上海应用物理研究所
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