Effects of serum proteins on intracellular uptake and cytotoxicity of carbon nanoparticles
文献类型：期刊论文
| 作者 | Zhu, Y(诸颖） ; Li, WX（李文新） ; Li, QN（李晴暖） ; Li, YG（李宇国） ; Li, YF ; Zhang, XY ; Huang, Q |
| 刊名 | CARBON
 |
| 出版日期 | 2009 |
| 卷号 | 47期号:5页码:8 |
| ISSN号 | 0008-6223 |
| 通讯作者 | Li, WX (reprint author), Chinese Acad Sci, Lab Nanobiol & Med, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China |
| 英文摘要 | To explore the effects of the novel properties of carbon nanoparticles (CNPs) on cytotoxicity, the adsorption of serum proteins in cell culture medium on multi-walled carbon nanotubes and three kinds of carbon blacks was investigated. The uptake of CNPs by Hela cells was measured quantitatively using (99m)Tc radionuclide labeling and tracing techniques, and the dependence of CNPs uptake on serum proteins was examined. The cytotoxicity of CNPs in medium with and without serum was assayed by the MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] method. It was found that cellular uptake was much higher in cells exposed to CNPs in serum-free culture medium than those in culture medium with serum. Serum proteins adsorbed on CNPs attenuated the inherent cytotoxicity of CNPs, and the extent of toxicity attenuation increased with increasing amounts of serum proteins adsorbed on CNPs. The possible reasons responsible for the considerable influence of serum proteins on cytotoxicity were indicated. (C) 2009 Elsevier Ltd. All rights reserved. |
| 学科主题 | Chemistry; Materials Science |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000264945600021 |
| 公开日期 | 2012-04-11 |
| 源URL | [http://ir.sinap.ac.cn/handle/331007/6904]  |
| 专题 | 上海应用物理研究所_中科院上海应用物理研究所2004-2010年 |
| 推荐引用方式 GB/T 7714 | Zhu, Y,Li, WX,Li, QN,et al. Effects of serum proteins on intracellular uptake and cytotoxicity of carbon nanoparticles[J]. CARBON,2009,47(5):8. |
| APA | Zhu, Y.,Li, WX.,Li, QN.,Li, YG.,Li, YF.,...&Huang, Q.(2009).Effects of serum proteins on intracellular uptake and cytotoxicity of carbon nanoparticles.CARBON,47(5),8. |
| MLA | Zhu, Y,et al."Effects of serum proteins on intracellular uptake and cytotoxicity of carbon nanoparticles".CARBON 47.5(2009):8. |
入库方式： OAI收割
来源：上海应用物理研究所
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