Enzymatic Digestion of Single DNA Molecules Anchored on Nanogold-Modified Surfaces
文献类型：期刊论文
| 作者 | Lu, JH（吕军鸿） ; Ye, M（叶鸣） ; Duan, N ; Li, B（李宾） |
| 刊名 | NANOSCALE RESEARCH LETTERS
 |
| 出版日期 | 2009 |
| 卷号 | 4期号:9页码:6 |
| 关键词 | Gold nanoparticles Mica DNA Atomic force microscopy Dip-Pen Nanolithography |
| ISSN号 | 1931-7573 |
| 通讯作者 | Li, B (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, POB 800-204, Shanghai 201800, Peoples R China |
| 英文摘要 | To study enzyme-DNA interactions at single molecular level, both the attachment points and the immediate surroundings of surfaces must be carefully considered such that they do not compromise the structural information and biological properties of the sample under investigation. The present work demonstrates the feasibility of enzymatic digestion of single DNA molecules attached to nanoparticle-modified surfaces. With Nanogold linking DNA to the mica surface by electrostatic interactions, advantageous conditions with fewer effects on the length and topography of DNA are obtained, and an appropriate environment for the activities of DNA is created. We demonstrate that by using Dip-Pen Nanolithography, individual DNA molecules attached to modified mica surfaces can be efficiently digested by DNase I. |
| 学科主题 | Science & Technology - Other Topics; Materials Science; Physics |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000269013100010 |
| 公开日期 | 2012-04-11 |
| 源URL | [http://ir.sinap.ac.cn/handle/331007/6911]  |
| 专题 | 上海应用物理研究所_中科院上海应用物理研究所2004-2010年 |
| 推荐引用方式 GB/T 7714 | Lu, JH,Ye, M,Duan, N,et al. Enzymatic Digestion of Single DNA Molecules Anchored on Nanogold-Modified Surfaces[J]. NANOSCALE RESEARCH LETTERS,2009,4(9):6. |
| APA | Lu, JH,Ye, M,Duan, N,&Li, B.(2009).Enzymatic Digestion of Single DNA Molecules Anchored on Nanogold-Modified Surfaces.NANOSCALE RESEARCH LETTERS,4(9),6. |
| MLA | Lu, JH,et al."Enzymatic Digestion of Single DNA Molecules Anchored on Nanogold-Modified Surfaces".NANOSCALE RESEARCH LETTERS 4.9(2009):6. |
入库方式： OAI收割
来源：上海应用物理研究所
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