Improved enzyme immobilization for enhanced bioelectrocatalytic activity of glucose sensor
文献类型：期刊论文
| 作者 | Wang, YT ; Yu, L ; Zhu, ZQ ; Zhang, J ; Zhu, JZ ; Fan, CH(樊春海） |
| 刊名 | SENSORS AND ACTUATORS B-CHEMICAL
 |
| 出版日期 | 2009 |
| 卷号 | 136期号:2页码:6 |
| 关键词 | Glucose sensor Glucose oxidase MWNTs ZnO particles Synergic action |
| ISSN号 | 0925-4005 |
| 通讯作者 | Zhu, JZ (reprint author), E China Normal Univ, Sch Informat Sci & Technol, 3663 N Zhongshan Rd, Shanghai 200062, Peoples R China |
| 英文摘要 | In this work, we report the development of a highly sensitive and stable glucose sensor based on the synergetic effect of multi-walled carbon nanotubes (MWNTs) and ZnO nanoparticles. Since the isoelectric point (IEP) of glucose oxidase (GOx) is significantly different from that of ZnO nanoparticles, GOx was electrostatically bound to ZnO nanoparticles decorated onto a negatively charged MWNTs layer at the electrode surface via VLS growth. A cationic polydiallyldimethylammonium chloride (PDDA) layer was further coated onto the GOx-contained ZnO nanoparticle layer to prevent the leakage of GOx. This unique multi-layer structure (PDDA/GOx/ZnO/MWNTs) provided a favorable microenvironment to maintain the bioactivity of GOx, which led to rapid amperometric response toward glucose. By loading 0.5-U GOx at the sensor surface, we obtained a wide linear response range of 0.1-16mM for this sensor. High sensitivity of 50.2 mA cm(-2) M(-1) was obtained with increased loading of GOx (2.0U), leading to an even lower detection limit of 250 nM. This nanomaterials-based glucose sensor was highly sensitive and showed favorable stability over a relatively long-term storage (160 days). Importantly, we challenged our sensor with 100 human blood serum samples, and obtained consistent results with the classic spectrometric assay (correlation coefficient, 0.997). (c) 2009 Elsevier B.V. All rights reserved. |
| 学科主题 | Chemistry; Electrochemistry; Instruments & Instrumentation |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000264089600009 |
| 公开日期 | 2012-04-11 |
| 源URL | [http://ir.sinap.ac.cn/handle/331007/6937]  |
| 专题 | 上海应用物理研究所_中科院上海应用物理研究所2004-2010年 |
| 推荐引用方式 GB/T 7714 | Wang, YT,Yu, L,Zhu, ZQ,et al. Improved enzyme immobilization for enhanced bioelectrocatalytic activity of glucose sensor[J]. SENSORS AND ACTUATORS B-CHEMICAL,2009,136(2):6. |
| APA | Wang, YT,Yu, L,Zhu, ZQ,Zhang, J,Zhu, JZ,&Fan, CH.(2009).Improved enzyme immobilization for enhanced bioelectrocatalytic activity of glucose sensor.SENSORS AND ACTUATORS B-CHEMICAL,136(2),6. |
| MLA | Wang, YT,et al."Improved enzyme immobilization for enhanced bioelectrocatalytic activity of glucose sensor".SENSORS AND ACTUATORS B-CHEMICAL 136.2(2009):6. |
入库方式： OAI收割
来源：上海应用物理研究所
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