Sequence-specific DNA detection by using biocatalyzed electrochemiluminescence and non-fouling surfaces
文献类型：期刊论文
| 作者 | Zhang, LY(张兰勇） ; Li, D（李迪） ; Meng, WL ; Huang, Q ; Su, Y ; Wang, LH（王丽华） ; Song, SP（宋世平） ; Fan, CH（樊春海） |
| 刊名 | BIOSENSORS & BIOELECTRONICS
 |
| 出版日期 | 2009 |
| 卷号 | 25期号:2页码:5 |
| 关键词 | DNA sensor Enzyme Electrochemiluminescence Non-fouling |
| ISSN号 | 0956-5663 |
| 通讯作者 | Li, D (reprint author), Chinese Acad Sci, Phys Biol Lab, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China |
| 英文摘要 | We herein report a DNA sensor for the sequence-specific DNA detection by using glucose oxidase-based biocatalyzed electrochemiluminescence and non-fouling surfaces. In this design, a glucose oxidase-labeled sandwich-type DNA sensor was built on a non-fouling surfaces made of a mixed self-assembled monolayers (SAMs) incorporating thiolated oligonucleotides and oligo(ethylene glycol) (OEG) thiols (SH-DNA/OEG). The sequence-specific DNA sensing was accomplished by the electrochemiluminesce (ECL) signal of luminol with the in-situ generated H(2)O(2). The protein-resistant non-fouling surfaces significantly suppressed the non-specific adsorption of enzyme label on electrode and reduced the background noise of this sensor. This sensor was able to detect as little as 1 pM of target DNA in pure buffer matrix. In complicated biological fluids such as human serum, this non-fouling platform-based sensor also revealed superior performance over conventional sandwich-type DNA sensors with mercaptohexanol (MCH)-coated surfaces. This strategy combines the high sensitivity of enzymatic amplified ECL and protein-resistant non-fouling platform, which is expected to be extended to a range of biological detection. (C) 2009 Elsevier B.V. All rights reserved. |
| 学科主题 | Biophysics; Biotechnology & Applied Microbiology; Chemistry; Electrochemistry; Science & Technology - Other Topics |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000270764300017 |
| 公开日期 | 2012-04-11 |
| 源URL | [http://ir.sinap.ac.cn/handle/331007/7043]  |
| 专题 | 上海应用物理研究所_中科院上海应用物理研究所2004-2010年 |
| 推荐引用方式 GB/T 7714 | Zhang, LY,Li, D,Meng, WL,et al. Sequence-specific DNA detection by using biocatalyzed electrochemiluminescence and non-fouling surfaces[J]. BIOSENSORS & BIOELECTRONICS,2009,25(2):5. |
| APA | Zhang, LY.,Li, D.,Meng, WL.,Huang, Q.,Su, Y.,...&Fan, CH.(2009).Sequence-specific DNA detection by using biocatalyzed electrochemiluminescence and non-fouling surfaces.BIOSENSORS & BIOELECTRONICS,25(2),5. |
| MLA | Zhang, LY,et al."Sequence-specific DNA detection by using biocatalyzed electrochemiluminescence and non-fouling surfaces".BIOSENSORS & BIOELECTRONICS 25.2(2009):5. |
入库方式： OAI收割
来源：上海应用物理研究所
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