Characterization of a chestnut FLORICAULA/LEAFY homologous gene
文献类型：期刊论文
| 作者 | Liu, Tao1; Hu, Yun-qian2; Li, Xiao-xian2 |
| 刊名 | AFRICAN JOURNAL OF BIOTECHNOLOGY
 |
| 出版日期 | 2011-05-16 |
| 卷号 | 10期号:20页码:3978-3985 |
| 关键词 | Chestnut homologue FLORICAULA/LEAFY |
| ISSN号 | 1684-5315 |
| 通讯作者 | Liu, T (reprint author), Yunnan Agr Univ, Fac Agron & Biotechnol, Kunming 650201, Yunnan, Peoples R China |
| 英文摘要 | The FLORICAULA/LEAFY (FLO/LFY) homologues' genes are necessary for normal flower development and play a key role in diverse angiosperm species. In this paper, an orthologue of FLORICAULA/LEAFY, CmLFY (chestnut FLO/LFY), was isolated from the inflorescence of chestnut trees. Its expression was detected in various tissues. Furthermore, the flowering effectiveness of the gene was assessed with transgenic Arabidopsis. CmLFY protein showed a high degree of similarity to PEAFLO (78%), which is a homologue of FLO/LFY from pea. RT-PCR analysis showed that, CmLFY expressed at high levels in inflorescences, but not in young leaves, fruits or stems. The transgenic Arabidopsis with over-expressed CmLFY showed accelerated flowering, which supports that CmLFY encodes a functional orthologue of the FLORICAULA/LEAFY genes of angiosperms despite its sequence divergence. These results suggest that CmLFY is involved in inflorescence development in chestnut. |
| 学科主题 | Biotechnology & Applied Microbiology |
| 类目[WOS] | Biotechnology & Applied Microbiology |
| 研究领域[WOS] | Biotechnology & Applied Microbiology |
| 关键词[WOS] | FLORAL MERISTEM IDENTITY ; FLO/LFY HOMOLOG ; ARABIDOPSIS ; LEAFY ; FLOWER ; EXPRESSION ; CLONING ; OVEREXPRESSION ; INITIATION ; ORTHOLOG |
| 收录类别 | SCI |
| 资助信息 | Faculty of Agronomy and Biotechnology[SJCX09012]; National Natural Science Foundation of China (NSFC)[30800062] |
| 语种 | 英语 |
| WOS记录号 | WOS:000294612200004 |
| 公开日期 | 2012-04-10 |
| 源URL | [http://ir.kib.ac.cn/handle/151853/5309]  |
| 专题 | 昆明植物研究所_资源植物与生物技术所级重点实验室 |
| 作者单位 | 1.Yunnan Agr Univ, Fac Agron & Biotechnol, Kunming 650201, Yunnan, Peoples R China 2.Chinese Acad Sci, Kunming Inst Bot, Kunming 650204, Yunnan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Tao,Hu, Yun-qian,Li, Xiao-xian. Characterization of a chestnut FLORICAULA/LEAFY homologous gene[J]. AFRICAN JOURNAL OF BIOTECHNOLOGY,2011,10(20):3978-3985. |
| APA | Liu, Tao,Hu, Yun-qian,&Li, Xiao-xian.(2011).Characterization of a chestnut FLORICAULA/LEAFY homologous gene.AFRICAN JOURNAL OF BIOTECHNOLOGY,10(20),3978-3985. |
| MLA | Liu, Tao,et al."Characterization of a chestnut FLORICAULA/LEAFY homologous gene".AFRICAN JOURNAL OF BIOTECHNOLOGY 10.20(2011):3978-3985. |
入库方式： OAI收割
来源：昆明植物研究所
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