Tuning backbones and side-chains of cationic conjugated polymers for optical signal amplification of fluorescent DNA detection
文献类型：期刊论文
| 作者 | Huang, YQ ; Liu, XF ; Fan, QL ; Wang, LH ; Song, SP ; Wang, LH(王丽华） ; Fan, CH（樊春海） ; Huang, W |
| 刊名 | BIOSENSORS & BIOELECTRONICS
 |
| 出版日期 | 2009 |
| 卷号 | 24期号:10页码:6 |
| 关键词 | Cationic conjugated polymer DNA Amphiphilic Forster resonance energy transfer Signal amplification |
| ISSN号 | 0956-5663 |
| 通讯作者 | Fan, CH (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China |
| 英文摘要 | Three cationic conjugated polymers (CCPs) exhibiting different backbone geometries and charge densities were used to investigate how their conjugated backbone and side chain properties, together with the transitions of DNA amphiphilic properties, interplay in the CCP/DNA-C* (DNA-C*: fluorophore-labeled DNA) complexes to influence the optical signal amplification of fluorescent DNA detection based oil Forster resonance energy transfer (FRET). By examining the FRET efficiencies to dsDNA-C* (dsDNA: double-stranded DNA) and ssDNA-C* (ssDNA: single-stranded DNA) for each CCP, twisted Conjugated backbones and higher charge densities were proved to facilitate electrostatic attraction in CCP/dsDNA-C* complexes, and induced improved sensitivity to DNA hybridization. Especially, by using the CCP with twisted conjugated backbone and the highest charge density, a more than 7-fold higher efficiency of FRET to dsDNA-C* was found than to ssDNA-C*, indicating a high signal amplification for discriminating between dsDNA and ssDNA. By contrast, linear conjugated backbones and lower charge density were demonstrated to favor hydrophobic interactions in CCP/ssDNA-C* complexes. These findings provided guidelines for the design of novel sensitive CCP, which can be useful to recognize many other important DNA activities involving transitions of DNA amphiphilic properties like DNA hybridization, such as specific DNA binding with ions, some secondary OF tertiary structural changes of DNA, and so forth. (C) 2009 Elseviei B.V. All rights reserved. |
| 学科主题 | Biophysics; Biotechnology & Applied Microbiology; Chemistry; Electrochemistry; Science & Technology - Other Topics |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000267162200004 |
| 公开日期 | 2012-04-11 |
| 源URL | [http://ir.sinap.ac.cn/handle/331007/7106]  |
| 专题 | 上海应用物理研究所_中科院上海应用物理研究所2004-2010年 |
| 推荐引用方式 GB/T 7714 | Huang, YQ,Liu, XF,Fan, QL,et al. Tuning backbones and side-chains of cationic conjugated polymers for optical signal amplification of fluorescent DNA detection[J]. BIOSENSORS & BIOELECTRONICS,2009,24(10):6. |
| APA | Huang, YQ.,Liu, XF.,Fan, QL.,Wang, LH.,Song, SP.,...&Huang, W.(2009).Tuning backbones and side-chains of cationic conjugated polymers for optical signal amplification of fluorescent DNA detection.BIOSENSORS & BIOELECTRONICS,24(10),6. |
| MLA | Huang, YQ,et al."Tuning backbones and side-chains of cationic conjugated polymers for optical signal amplification of fluorescent DNA detection".BIOSENSORS & BIOELECTRONICS 24.10(2009):6. |
入库方式： OAI收割
来源：上海应用物理研究所
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