VISUALIZATION EX SITU OF SINGLE DNA MOLECULES INCUBATION: A FIRST STEP FOR QUANTITATIVE ANALYSIS ON MULTI-SITE DEGRADATION AND ENZYMATIC KINETICS
文献类型：期刊论文
| 作者 | Wang, XY ; Yang, HJ ; Wang, HB ; Wang, P ; Li, H |
| 刊名 | SURFACE REVIEW AND LETTERS
 |
| 出版日期 | 2009 |
| 卷号 | 16期号:1页码:7 |
| 关键词 | Visualization atomic force microscopy ex situ modified dynamic molecular combing DNase I incubation degradation |
| ISSN号 | 0218-625X |
| 通讯作者 | Wang, XY (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China |
| 英文摘要 | Herein, we showed a different approach to directly single-molecule level visualization of the degradation of DNA in vitro tests using DNase I incubation based on high-resolution AFM imaging ex situ with fine relocation nanotechnology. A method of nanomanipulation termed as "modified dynamic molecular combing" (MDMC) was used to pattern DNA samples for further degradation and enzymatic kinetics. This strategy is potentially able to quantitatively address the mechanical-induced kinetic profiles of multi-site degradation of individual DNA molecules with very stable tension and strong immobilization on a surface and discover the mechanochemistry. |
| 学科主题 | Chemistry; Physics |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000267266300013 |
| 公开日期 | 2012-04-11 |
| 源URL | [http://ir.sinap.ac.cn/handle/331007/7112]  |
| 专题 | 上海应用物理研究所_中科院上海应用物理研究所2004-2010年 |
| 推荐引用方式 GB/T 7714 | Wang, XY,Yang, HJ,Wang, HB,et al. VISUALIZATION EX SITU OF SINGLE DNA MOLECULES INCUBATION: A FIRST STEP FOR QUANTITATIVE ANALYSIS ON MULTI-SITE DEGRADATION AND ENZYMATIC KINETICS[J]. SURFACE REVIEW AND LETTERS,2009,16(1):7. |
| APA | Wang, XY,Yang, HJ,Wang, HB,Wang, P,&Li, H.(2009).VISUALIZATION EX SITU OF SINGLE DNA MOLECULES INCUBATION: A FIRST STEP FOR QUANTITATIVE ANALYSIS ON MULTI-SITE DEGRADATION AND ENZYMATIC KINETICS.SURFACE REVIEW AND LETTERS,16(1),7. |
| MLA | Wang, XY,et al."VISUALIZATION EX SITU OF SINGLE DNA MOLECULES INCUBATION: A FIRST STEP FOR QUANTITATIVE ANALYSIS ON MULTI-SITE DEGRADATION AND ENZYMATIC KINETICS".SURFACE REVIEW AND LETTERS 16.1(2009):7. |
入库方式： OAI收割
来源：上海应用物理研究所
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