Analysis of Deoxynucleotide in Plasmid Deoxyribonucleic Acid Using High Performance Liquid Chromatography-Inductively Coupled Plasma-Mass Spectrometry
文献类型：期刊论文
| 作者 | Liang, F(梁峰） ; Li, YL（李玉兰） ; Lu, WW ; Tan, MG（谈明光） ; Zhang, GL（张桂林） ; Li, Y（李燕） |
| 刊名 | CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
 |
| 出版日期 | 2008 |
| 卷号 | 36期号:8页码:1097 |
| ISSN号 | 0253-3820 |
| 通讯作者 | Li, YL (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China |
| 英文摘要 | An ion pair reverse phase high performance liquid chromatography (RP-HPLC) coupled with inductively coupled plasma mass spectrometry (ICP-MS) method has been developed for the determination of deoxyribonucleotide in plasmid DNA. Optimum separation conditions for the four deoxyribonucleotides Including 2'-deoxycytidine-5'-monophosphate (dCMP), 2'-deoxythymidine-5'-monophosphate (dCMP), 2'-deoxyguanosine-5'-monophosphate (dGMP) and 2'-deoxyadenosine-5'-monophosphate (dAMP) are obtained using a mobile phase containing 10 mmol/L ammonium acetate (pH 4.8) and 2.5% methanol. The hexapole collision/reaction cell technique (CCT) by adding O-2 is employed for the detection of (PO+)-P-31-O-16 to avoid potential interferences of polyatomic isobaric at m/z 31 by directly detecting P-31(+). By such HPLC-TCP-MS hyphenation technique, detection limits of four deoxyribonucleotides are 0.211 mu mol/L for dCMP, 0.204 mu mol/L for dTMP, 0.173 mu mol/L for dGMP and 0.225 mu mol/L for dAMP, respectively. This hyphenated HPLC-ICP-MS method has been successfully applied to the analysis of four deoxyribonucleotides in plasmid DNA, which contained 152.9 +/- 2.4 mu mol/L of dCMP, 228.2 +/- 4.0 mu mol/L of dTMP, 125.3 +/- 3.0 mu mol/L of dGMP and 222.9 +/- 3.3 mu mol/L of dAMP. |
| 学科主题 | Physics |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000260136800017 |
| 公开日期 | 2012-04-18 |
| 源URL | [http://ir.sinap.ac.cn/handle/331007/7648]  |
| 专题 | 上海应用物理研究所_中科院上海应用物理研究所2004-2010年 |
| 推荐引用方式 GB/T 7714 | Liang, F,Li, YL,Lu, WW,et al. Analysis of Deoxynucleotide in Plasmid Deoxyribonucleic Acid Using High Performance Liquid Chromatography-Inductively Coupled Plasma-Mass Spectrometry[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2008,36(8):1097. |
| APA | Liang, F,Li, YL,Lu, WW,Tan, MG,Zhang, GL,&Li, Y.(2008).Analysis of Deoxynucleotide in Plasmid Deoxyribonucleic Acid Using High Performance Liquid Chromatography-Inductively Coupled Plasma-Mass Spectrometry.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,36(8),1097. |
| MLA | Liang, F,et al."Analysis of Deoxynucleotide in Plasmid Deoxyribonucleic Acid Using High Performance Liquid Chromatography-Inductively Coupled Plasma-Mass Spectrometry".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 36.8(2008):1097. |
入库方式： OAI收割
来源：上海应用物理研究所
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