Effects of high-energy-pulse-electron beam radiation on biomacromolecules
文献类型：期刊论文
| 作者 | Zhu, H ; Xu, JZ ; Li, SQ ; Sun, XY ; Yao, SD（姚思德） ; Wang, SL |
| 刊名 | SCIENCE IN CHINA SERIES B-CHEMISTRY
 |
| 出版日期 | 2008 |
| 卷号 | 51期号:1页码:86 |
| ISSN号 | 1006-9291 |
| 通讯作者 | Wang, SL (reprint author), Tongji Univ, Sch Life Sci & Technol, Shanghai 200092, Peoples R China |
| 英文摘要 | To study the molecular mechanism of high mutation frequency induced by high-energy-pulse-electron (HEPE) beam radiation, the effects of HEPE radiation on yeast cells, plasma membrane, plasmid DNA, and protein activity were investigated by means of cell counting, gel electrophoresis, AO/EB double fluorescent staining, etc. The results showed that the viability of yeast cells declined statistically with increase of absorbed doses. The half lethal dose (LD50) was 134 Gy. HEPE beam radiation had little influence on the function of plasma membrane and protein, while it could induce much DNA damage of single strand breaks (SSB) and double strand breaks (DSB) that were required for gene mutation. The G-value for DSB formation of HEPE beam radiation in aqueous solution was 5.7 times higher than that caused by Co-60 gamma rays. HEPE can be a new effective method for induced mutation breeding and deserves further research in the future. |
| 学科主题 | Computer Science; Engineering; Robotics |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000251972200013 |
| 公开日期 | 2012-04-18 |
| 源URL | [http://ir.sinap.ac.cn/handle/331007/7709]  |
| 专题 | 上海应用物理研究所_中科院上海应用物理研究所2004-2010年 |
| 推荐引用方式 GB/T 7714 | Zhu, H,Xu, JZ,Li, SQ,et al. Effects of high-energy-pulse-electron beam radiation on biomacromolecules[J]. SCIENCE IN CHINA SERIES B-CHEMISTRY,2008,51(1):86. |
| APA | Zhu, H,Xu, JZ,Li, SQ,Sun, XY,Yao, SD,&Wang, SL.(2008).Effects of high-energy-pulse-electron beam radiation on biomacromolecules.SCIENCE IN CHINA SERIES B-CHEMISTRY,51(1),86. |
| MLA | Zhu, H,et al."Effects of high-energy-pulse-electron beam radiation on biomacromolecules".SCIENCE IN CHINA SERIES B-CHEMISTRY 51.1(2008):86. |
入库方式： OAI收割
来源：上海应用物理研究所
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