Investigation on the morphology of precipitated chemicals from TE buffer on solid substrates
文献类型：期刊论文
| 作者 | Wang, HB(王化斌） ; Zhang, LJ ; Zhang, F ; An, HJ ; Chen, SM（陈仕谋） ; Li, H ; Wang, P ; Wang, XY ; Wang, Y ; Yang, HJ |
| 刊名 | SURFACE REVIEW AND LETTERS
 |
| 出版日期 | 2007 |
| 卷号 | 14期号:6页码:1121 |
| ISSN号 | 0218-625X |
| 通讯作者 | Wang, HB (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China |
| 英文摘要 | Buffer is often involved in the biological studies on a surface with the scanning probe microscopy and the remnant of the salts of buffer may affect the correct interpretation of the experimental results. However, the knowledge on the performance of chemicals of buffer on a substrate is still very poor. TE solution (Tris-HCl, NaCl, and ethylene diamine tetraacetic acid (EDTA)) is a widely used buffer in stocking biological molecules. Herein, we report the performance of the precipitated chemicals from TE solution on two typical substrates with several commonly used sample preparation methods. The results showed that the chemicals in TE solution could self-organize into parallel nano. laments on hydrophobic highly oriented pyrolitic graphite (HOPG) surface by blotting the shortly incubated solution droplet from the substrate or by drying the diluted solution droplet naturally. In contrast, no such special structures were observed on hydrophilic mica using the same methods. By imaging in TE solutions, no special structures were found on either the HOPG or the mica. Moreover, the effects of the concentration of chemicals of TE buffer on the morphology of precipitated chemicals were also investigated. |
| 学科主题 | Chemistry; Science & Technology - Other Topics; Materials Science |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000252522100016 |
| 公开日期 | 2012-04-18 |
| 源URL | [http://ir.sinap.ac.cn/handle/331007/7757]  |
| 专题 | 上海应用物理研究所_中科院上海应用物理研究所2004-2010年 |
| 推荐引用方式 GB/T 7714 | Wang, HB,Zhang, LJ,Zhang, F,et al. Investigation on the morphology of precipitated chemicals from TE buffer on solid substrates[J]. SURFACE REVIEW AND LETTERS,2007,14(6):1121. |
| APA | Wang, HB.,Zhang, LJ.,Zhang, F.,An, HJ.,Chen, SM.,...&Yang, HJ.(2007).Investigation on the morphology of precipitated chemicals from TE buffer on solid substrates.SURFACE REVIEW AND LETTERS,14(6),1121. |
| MLA | Wang, HB,et al."Investigation on the morphology of precipitated chemicals from TE buffer on solid substrates".SURFACE REVIEW AND LETTERS 14.6(2007):1121. |
入库方式： OAI收割
来源：上海应用物理研究所
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