Mechanism of the interaction between Au nanoparticles and polymerase in nanoparticle PCR
文献类型：期刊论文
| 作者 | Mi, LJ(米丽娟） ; Zhu, HP ; Zhang, XD ; Hu, J（胡钧） ; Fan, CH（樊春海） |
| 刊名 | CHINESE SCIENCE BULLETIN
 |
| 出版日期 | 2007 |
| 卷号 | 52期号:17页码:2345 |
| ISSN号 | 1001-6538 |
| 通讯作者 | Hu, J (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China |
| 英文摘要 | Nanoparticle PCR is a novel method to optimize DNA amplification. It performs well in improving specificity, enhancing sensitivity and speed. Several mechanisms were proposed in previous studies: one was based on the interaction between gold nanoparticles (AuNPs) and DNA while the other was attributed to the heat transfer property of AuNPs. In this paper, we propose that the interaction between AuNPs and DNA polymerase can significantly influence PCR. First, the addition of DNA polymerase can eliminate the inhibitory effects of excess AuNPs. Second, the addition of AuNPs will increase yield of the desired PCR product and make the optimum concentration of DNA polymerase move to higher value. Third, while excess polymerase might inhibit amplification efficiency, AuNPs can reverse this process and the yield of PCR amplification. Based on these results we propose a possible mechanism that AuNPs might modulate the activity of polymerase and improve PCR amplification. |
| 学科主题 | Polymer Science |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000249381100008 |
| 公开日期 | 2012-04-18 |
| 源URL | [http://ir.sinap.ac.cn/handle/331007/7777]  |
| 专题 | 上海应用物理研究所_中科院上海应用物理研究所2004-2010年 |
| 推荐引用方式 GB/T 7714 | Mi, LJ,Zhu, HP,Zhang, XD,et al. Mechanism of the interaction between Au nanoparticles and polymerase in nanoparticle PCR[J]. CHINESE SCIENCE BULLETIN,2007,52(17):2345. |
| APA | Mi, LJ,Zhu, HP,Zhang, XD,Hu, J,&Fan, CH.(2007).Mechanism of the interaction between Au nanoparticles and polymerase in nanoparticle PCR.CHINESE SCIENCE BULLETIN,52(17),2345. |
| MLA | Mi, LJ,et al."Mechanism of the interaction between Au nanoparticles and polymerase in nanoparticle PCR".CHINESE SCIENCE BULLETIN 52.17(2007):2345. |
入库方式： OAI收割
来源：上海应用物理研究所
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