Study on the interaction of colloidal gold with Taq DNA polymerase
文献类型：期刊论文
| 作者 | Zhu, HP(朱红平） ; Mi, LJ（米丽娟） ; Chen, SM（陈仕谋） ; Wang, WF（王文锋） ; Yao, SD（姚思德） |
| 刊名 | CHINESE JOURNAL OF CHEMISTRY
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| 出版日期 | 2007 |
| 卷号 | 25期号:9页码:1233 |
| ISSN号 | 1001-604X |
| 通讯作者 | Zhu, HP (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China |
| 英文摘要 | The interaction of colloidal gold with Taq DNA polymerase (Taq) was investigated in this study. Taq-gold conjugate was formed by adding the enzyme to the colloidal gold solution, as evidenced by UV-Vis spectroscopy, X-ray photoelectron spectroscopy, and photon cross correlation spectroscopy measurements. The conjugate was further characterized by transmission electron microscopy. It was found that the Taq-gold conjugate particles were still spherical and well-dispersed. The influence of gold nanoparticles on the bioactivity of Taq was studied by analyzing the yield of the polymerase chain reaction amplification. Results indicated that the enzymatic activity of Taq decreased after interaction with the colloidal gold. |
| 学科主题 | Chemistry |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000249473600003 |
| 公开日期 | 2012-04-18 |
| 源URL | [http://ir.sinap.ac.cn/handle/331007/7842]  |
| 专题 | 上海应用物理研究所_中科院上海应用物理研究所2004-2010年 |
| 推荐引用方式 GB/T 7714 | Zhu, HP,Mi, LJ,Chen, SM,et al. Study on the interaction of colloidal gold with Taq DNA polymerase[J]. CHINESE JOURNAL OF CHEMISTRY,2007,25(9):1233. |
| APA | Zhu, HP,Mi, LJ,Chen, SM,Wang, WF,&Yao, SD.(2007).Study on the interaction of colloidal gold with Taq DNA polymerase.CHINESE JOURNAL OF CHEMISTRY,25(9),1233. |
| MLA | Zhu, HP,et al."Study on the interaction of colloidal gold with Taq DNA polymerase".CHINESE JOURNAL OF CHEMISTRY 25.9(2007):1233. |
入库方式： OAI收割
来源：上海应用物理研究所
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