Trypsin-Gold Nanoparticle Conjugates: Binding, Enzymatic Activity, and Stability
文献类型：期刊论文
| 作者 | Lv, M ; Zhu, EG(朱恩高） ; Su, YY ; Li, QN（李晴暖） ; Li, WX（李文新） ; Zhao, Y（赵云） ; Huang, Q（黄庆） |
| 刊名 | PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY
 |
| 出版日期 | 2009 |
| 卷号 | 39期号:4页码:429 |
| ISSN号 | 1082-6068 |
| 通讯作者 | Huang, Q (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China |
| 英文摘要 | Nanomaterials may interact with biomolecules in various ways and change their bioactivities. Here, we report on how gold nanoparticles (AuNPs) affect a most important protease, trypsin. After simple mixing of the trypsin and AuNPs solution the average diameter is 20nm under enzyme friendly conditions (pH 8.0); the bond between trypsin and AuNPs was detected by UV-vis spectroscopy. The further protease assay of trypsin, before and after mixing with the AuNPs solution, pointed out an improved performance in terms of enzyme activity and stability. |
| 学科主题 | Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000269668300008 |
| 公开日期 | 2012-04-18 |
| 源URL | [http://ir.sinap.ac.cn/handle/331007/7868]  |
| 专题 | 上海应用物理研究所_中科院上海应用物理研究所2004-2010年 |
| 推荐引用方式 GB/T 7714 | Lv, M,Zhu, EG,Su, YY,et al. Trypsin-Gold Nanoparticle Conjugates: Binding, Enzymatic Activity, and Stability[J]. PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY,2009,39(4):429. |
| APA | Lv, M.,Zhu, EG.,Su, YY.,Li, QN.,Li, WX.,...&Huang, Q.(2009).Trypsin-Gold Nanoparticle Conjugates: Binding, Enzymatic Activity, and Stability.PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY,39(4),429. |
| MLA | Lv, M,et al."Trypsin-Gold Nanoparticle Conjugates: Binding, Enzymatic Activity, and Stability".PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY 39.4(2009):429. |
入库方式： OAI收割
来源：上海应用物理研究所
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