Spectrophotometric detection of lead(II) ion using unimolecular peroxidase-like deoxyribozyme
文献类型：期刊论文
| 作者 | Wang Y ; Wang JA ; Yang F ; Yang XR |
| 刊名 | microchimica acta
 |
| 出版日期 | 2010 |
| 卷号 | 171期号:1-2页码:195-201 |
| 关键词 | PLASMA-MASS SPECTROMETRY ANODIC-STRIPPING VOLTAMMETRY ATOMIC-ABSORPTION-SPECTROMETRY COLORIMETRIC DETECTION EMISSION-SPECTROMETRY SPECIATION ANALYSIS TELOMERASE ACTIVITY GOLD NANOPARTICLES HYDRIDE GENERATION ISOTOPE-DILUTION |
| ISSN号 | 0026-3672 |
| 通讯作者 | yang xr |
| 中文摘要 | deoxyribonucleic acid (dna) can form g-quadruplexes in the presence of certain metal ions. these play a functional role in a variety of biological process. a dna 17-mer (pw17) was previously reported to bind hemin in the presence of excess potassium ion. the resulting stabilized g-quadruplex-hemin complex exhibits peroxidase-like activity. however, this activity is lost in the presence of one equivalent of pb2+ ion. we exploit this property in a method for spectrophotometric detection of pb2+. inhibition by pb2+ ion is reflected by a change in the soret band of hemin and a sharp reduction in the catalytic activity towards hydrogen peroxide-mediated chromogenic oxidation of 2,2'-azino-bis(3-ethylbenzothiazoline)-6-sulfonate. the new method enables pb2+ to be detected in the concentration range from 0.05 to 1.2 mu m, with a detection limit of 27 nm. the assay shows high selectivity over other metal ions. it was successfully used to determine pb2+ in water samples. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000281669900026 |
| 公开日期 | 2012-04-26 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/43477]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Wang Y,Wang JA,Yang F,et al. Spectrophotometric detection of lead(II) ion using unimolecular peroxidase-like deoxyribozyme[J]. microchimica acta,2010,171(1-2):195-201. |
| APA | Wang Y,Wang JA,Yang F,&Yang XR.(2010).Spectrophotometric detection of lead(II) ion using unimolecular peroxidase-like deoxyribozyme.microchimica acta,171(1-2),195-201. |
| MLA | Wang Y,et al."Spectrophotometric detection of lead(II) ion using unimolecular peroxidase-like deoxyribozyme".microchimica acta 171.1-2(2010):195-201. |
入库方式： OAI收割
来源：长春应用化学研究所
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