Parallel G-Quadruplex-Specific Fluorescent Probe for Monitoring DNA Structural Changes and Label-Free Detection of Potassium Ion
文献类型：期刊论文
| 作者 | Li T ; Wang EK ; Dong SJ |
| 刊名 | analytical chemistry
 |
| 出版日期 | 2010 |
| 卷号 | 82期号:18页码:7576-7580 |
| 关键词 | PROTOPORPHYRIN-IX AQUEOUS-SOLUTION COLORIMETRIC DETECTION PORPHYRINS BINDING DNAZYME SENSOR RECOGNITION APTAMERS COMPLEX |
| ISSN号 | 0003-2700 |
| 通讯作者 | wang ek |
| 中文摘要 | here we demonstrate an anionic porphyrin, protoporphyrin ix (ppix), as a parallel g-quadruplex-specific fluorescent probe for monitoring dna structural changes and utilize it to develop a dna-based k+ sensor. the interactions of ppix with different dna structures in k+ or na+ solution are investigated by using circular dichroism, fluorescence, and uv-vis spectroscopy. the observations reveal that ppix has an similar to 100-fold selectivity for parallel g-quadruplexes against duplexes and antiparallel g-quadruplexes. meanwhile, the fluorescence intensity of ppix increases by over 10-fold upon binding to parallel g-quadruplexes. on the basis of the selectivity and fluorescence property of ppix, we introduce a facile, label-free approach to monitoring dna structural changes via fluorescence signal read-out that is tuned by ppix binding and release. to illustrate it, we utilize ppix and a g-rich dna ps2.m to construct a fluorescent k+ sensor based on an antiparallel-to-parallel conformation transition of the g-quadruplex. ps2.m adopts an antiparallel quadruplex structure in na+ solution, whereas it gradually converts into a parallel g-quadruplex upon addition of increasing k+. this conformational change is indicated by a sharp increase in the fluorescence intensity of ppix, owing to the good ability of ppix to discriminate parallel g-quadruplexes from antiparallel ones. even in the presence of 100 mm na+, such a "turn-on" fluorescent sensor can respond to low concentrations of k+, with a limit of detection (0.5 mm) for k+ analysis. in addition, this sensor exhibits a high selectivity for k+ over other common metal ions, which ensures its practical applications to real samples. these results reveal that ppix is promising for use as a specific dna structural probe in sensing applications. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000281710900008 |
| 公开日期 | 2012-04-26 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/43481]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Li T,Wang EK,Dong SJ. Parallel G-Quadruplex-Specific Fluorescent Probe for Monitoring DNA Structural Changes and Label-Free Detection of Potassium Ion[J]. analytical chemistry,2010,82(18):7576-7580. |
| APA | Li T,Wang EK,&Dong SJ.(2010).Parallel G-Quadruplex-Specific Fluorescent Probe for Monitoring DNA Structural Changes and Label-Free Detection of Potassium Ion.analytical chemistry,82(18),7576-7580. |
| MLA | Li T,et al."Parallel G-Quadruplex-Specific Fluorescent Probe for Monitoring DNA Structural Changes and Label-Free Detection of Potassium Ion".analytical chemistry 82.18(2010):7576-7580. |
入库方式： OAI收割
来源：长春应用化学研究所
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