A new method for multi-site-directed mutagenesis
文献类型：期刊论文
| 作者 | Tian J ; Liu QO ; Dong S ; Qiao XF ; Ni JZ |
| 刊名 | analytical biochemistry
 |
| 出版日期 | 2010 |
| 卷号 | 406期号:1页码:83-85 |
| 关键词 | HOMOLOGOUS RECOMBINATION PCR COMBINATION EFFICIENCY MUTATIONS PROTOCOL DELETION STRATEGY SINGLE STEP |
| ISSN号 | 0003-2697 |
| 通讯作者 | ni jz |
| 中文摘要 | a modified method for multi-site-directed mutagenesis was developed here based on polymerase chain reaction (pcr), dpni digestion, and overlap extension. it needs only methylated plasmids obtained by dam methyltransferase or plasmids from dam(+) escherichia coil containing target gene. the procedure consists of pcr. dpni digestion, overlap extension pcr, and plasmid transformation. the method was developed for multi-site-directed mutagenesis, including close proximity of mutation sites. it does not require 5'-phosphorylated primers and ligation and, thus, significantly simplifies the routine work and reduces the experimental cost for multi-site-directed mutagenesis. (c) 2010 elsevier inc. all rights reserved. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000281508000013 |
| 公开日期 | 2012-04-26 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/43496]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Tian J,Liu QO,Dong S,et al. A new method for multi-site-directed mutagenesis[J]. analytical biochemistry,2010,406(1):83-85. |
| APA | Tian J,Liu QO,Dong S,Qiao XF,&Ni JZ.(2010).A new method for multi-site-directed mutagenesis.analytical biochemistry,406(1),83-85. |
| MLA | Tian J,et al."A new method for multi-site-directed mutagenesis".analytical biochemistry 406.1(2010):83-85. |
入库方式： OAI收割
来源：长春应用化学研究所
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