Developing oligonucleotide microarray-based resonance light scattering assay for DNA detection on the PAMAM dendrimer modified surface
文献类型：期刊论文
| 作者 | Li XM ; Gao JQ ; Liu DJ ; Wang ZX |
| 刊名 | analytical methods
 |
| 出版日期 | 2010 |
| 卷号 | 2期号:8页码:1008-1012 |
| 关键词 | GOLD NANOPARTICLE PROBES PROTEIN MICROARRAYS SILVER ENHANCEMENT GLASS SLIDES NUCLEIC-ACID QUANTUM-DOT PARTICLE SYSTEMS BINDING ARRAYS |
| ISSN号 | 1759-9660 |
| 通讯作者 | wang zx |
| 中文摘要 | here, a three-dimensional (3d) oligonucleotide microarray-based resonance light scattering (rls) assay has been developed for dna detection. oligonucleotide conjugated gold nanoparticles are used as rls probes for labeling the detection system, followed by silver deposition for signal enhancement. the 3d oligonucleotide microarray is fabricated on the home-made fourth-generation (g4) nh2-terminated poly(amidoamine) dendrimers (pamam) modified substrate. after activated by glutaraldehyde (ga), the pamam modified substrate enables to react with nh2-modified oligonucleotide with highly efficiency and generates highly signal-to-background ratio. this assay makes it possible to discriminate an oligonucleotide sequence from the target with single nucleotide mismatch with high selectivity. and detection sensitivity of the 3d microarray is nearly 3-fold higher than that of two-dimensional (2d) microarray which is fabricated on the commercial planar slide. the discrimination between g : t and c : t mismatch as well as the mismatch sites, a particularly difficult task for microarray-based assays, has also evaluated. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000281289400003 |
| 公开日期 | 2012-04-26 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/43508]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Li XM,Gao JQ,Liu DJ,et al. Developing oligonucleotide microarray-based resonance light scattering assay for DNA detection on the PAMAM dendrimer modified surface[J]. analytical methods,2010,2(8):1008-1012. |
| APA | Li XM,Gao JQ,Liu DJ,&Wang ZX.(2010).Developing oligonucleotide microarray-based resonance light scattering assay for DNA detection on the PAMAM dendrimer modified surface.analytical methods,2(8),1008-1012. |
| MLA | Li XM,et al."Developing oligonucleotide microarray-based resonance light scattering assay for DNA detection on the PAMAM dendrimer modified surface".analytical methods 2.8(2010):1008-1012. |
入库方式： OAI收割
来源：长春应用化学研究所
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