Prefractionation Method to Separate Proteomic Proteins into Multigroups by One-step Extraction
文献类型：期刊论文
| 作者 | Bai HX ; Liu XH ; Yang F ; Yang XR ; Wang EK |
| 刊名 | chemical journal of chinese universities-chinese
 |
| 出版日期 | 2010 |
| 卷号 | 31期号:7页码:1314-1321 |
| 关键词 | AQUEOUS 2-PHASE SYSTEMS MASS-SPECTROMETRY IDENTIFICATION HUMAN PLASMA PROTEOME 2-DIMENSIONAL ELECTROPHORESIS SAMPLE PREFRACTIONATION CHROMATOGRAPHY FRACTIONATION MODEL PURIFICATION PARTITION |
| ISSN号 | 0251-0790 |
| 通讯作者 | bai hx |
| 中文摘要 | for the first time, an aqueous two-phase system (atps) composed of n-propanol, nacl and water was applied in prefractionating proteomic proteins. in this work, ethanol, n-propanol and several commonly used inorganic salts were attempted to develop the atps. the atps composed of n-propanol, nacl and water was developed finally. subsequently, the effects of ph, salt concentration, n-propanol volume and protein concentration on the atps' separation effectiveness for proteins were studied. protein samples were prefractionated by the atps and then analyzed by gel electrophoresis. the results proved that the prefractionation method could separate proteomic proteins into three groups by one-step extraction, and there was no need for separating target proteins from the phase-forming reagents prior to gel electrophoresis analysis. simplicity, rapidity and low cost are the highlight attractiveness of the prefractionation method. it also has advantages of biocompability, potential for continuous operation, no requirement for sophisticated instrumentations and being easy or no need for separating the target proteins from phase-forming reagents prior to electrophoresis analysis. the prefractionation method has great significance in proteomics and methodology. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 中文 |
| WOS记录号 | WOS:000280429500007 |
| 公开日期 | 2012-04-26 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/43574]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Bai HX,Liu XH,Yang F,et al. Prefractionation Method to Separate Proteomic Proteins into Multigroups by One-step Extraction[J]. chemical journal of chinese universities-chinese,2010,31(7):1314-1321. |
| APA | Bai HX,Liu XH,Yang F,Yang XR,&Wang EK.(2010).Prefractionation Method to Separate Proteomic Proteins into Multigroups by One-step Extraction.chemical journal of chinese universities-chinese,31(7),1314-1321. |
| MLA | Bai HX,et al."Prefractionation Method to Separate Proteomic Proteins into Multigroups by One-step Extraction".chemical journal of chinese universities-chinese 31.7(2010):1314-1321. |
入库方式： OAI收割
来源：长春应用化学研究所
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