Locating the Band III protein in quasi-native cell membranes
文献类型:期刊论文
作者 | Shan YP ; Wang ZY ; Hao XA ; Shang X ; Cai MJ ; Jiang JG ; Fang XX ; Wang HD ; Tang ZY |
刊名 | analytical methods
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出版日期 | 2010 |
卷号 | 2期号:7页码:805-808 |
关键词 | ATOMIC-FORCE MICROSCOPY ANION-TRANSPORT PROTEIN ERYTHROCYTE-MEMBRANE DOMAIN SHAPE DNA LOCALIZATION FRAGMENTS CFTR |
ISSN号 | 1759-9660 |
通讯作者 | wang hd |
中文摘要 | band iii is a key protein for the structure and function of red blood cell membranes. to date, the distribution and morphology of band iii in cell membranes is still unclear because of limited approaches. we applied topography and recognition imaging microscopy (trec), which extends the application of atomic force microscopy (afm) to recognize a single molecule in a biological complex, to visually locate a single band iii protein in quasi-native cell membranes by anti-band iii-functionalized afm tips under physiological conditions. the band iii proteins are well distributed in the inner leaflet of cell membranes. the height of the whole band iii protein in cell membranes is in the range of 9-13 nm. the unbinding force between band iii in the membrane and anti-band iii on the afm tip is about 70 pn with the loading rate at 40 nn/s. our result is significant in revealing the location and morphology of band iii in the inner cell membrane at the molecular level. |
收录类别 | SCI收录期刊论文 |
语种 | 英语 |
WOS记录号 | WOS:000280415500003 |
公开日期 | 2012-04-26 |
源URL | [http://ir.ciac.jl.cn/handle/322003/43576] ![]() |
专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
推荐引用方式 GB/T 7714 | Shan YP,Wang ZY,Hao XA,et al. Locating the Band III protein in quasi-native cell membranes[J]. analytical methods,2010,2(7):805-808. |
APA | Shan YP.,Wang ZY.,Hao XA.,Shang X.,Cai MJ.,...&Tang ZY.(2010).Locating the Band III protein in quasi-native cell membranes.analytical methods,2(7),805-808. |
MLA | Shan YP,et al."Locating the Band III protein in quasi-native cell membranes".analytical methods 2.7(2010):805-808. |
入库方式: OAI收割
来源:长春应用化学研究所
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