A novel dot-blot DNAzyme-linked aptamer assay for protein detection
文献类型：期刊论文
| 作者 | Zhu JB ; Li T ; Hu JM ; Wang EK |
| 刊名 | analytical and bioanalytical chemistry
 |
| 出版日期 | 2010 |
| 卷号 | 397期号:7页码:2923-2927 |
| 关键词 | COLORIMETRIC DETECTION IMMUNOSORBENT-ASSAY RAPID DETECTION DNA THROMBIN LABEL COMPLEX |
| ISSN号 | 1618-2642 |
| 通讯作者 | wang ek |
| 中文摘要 | in this work, a novel dot-blot dnazyme-linked aptamer assay (dlaa) for protein detection is developed with thrombin as a model protein. a peroxidase-like dnazyme which serves as the catalytic label is tethered to a 15-mer thrombin-binding aptamer to form a label-free dnazyme-linked aptamer probe. based on specific interaction of the aptamer with target protein immobilized on nitrocellulose membrane, a dnazyme layer is introduced onto the membrane. the dnazyme can catalyze the h2o2-mediated oxidation of 3,3',5,5'-tetramethylbenzidine to produce a colored insoluble product that is apt to be adsorbed onto the nitrocellulose membrane. as a result, blue dots appear on the membrane, in contrast to the colorless background. as the concentration of thrombin increases, the color of dots gets deep. such a protein concentration-dependent color change can be quantified via an image-processing software, with a detection limit of 0.6 mu m. furthermore, this assay has been applied successfully to the detection of thrombin in biological samples (e.g., human serum), indicating its practicality for bioanalysis. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000280122100029 |
| 公开日期 | 2012-04-26 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/43588]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Zhu JB,Li T,Hu JM,et al. A novel dot-blot DNAzyme-linked aptamer assay for protein detection[J]. analytical and bioanalytical chemistry,2010,397(7):2923-2927. |
| APA | Zhu JB,Li T,Hu JM,&Wang EK.(2010).A novel dot-blot DNAzyme-linked aptamer assay for protein detection.analytical and bioanalytical chemistry,397(7),2923-2927. |
| MLA | Zhu JB,et al."A novel dot-blot DNAzyme-linked aptamer assay for protein detection".analytical and bioanalytical chemistry 397.7(2010):2923-2927. |
入库方式： OAI收割
来源：长春应用化学研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。