Nucleic acid G-quadruplex based label-free fluorescence turn-on potassium selective sensing
文献类型：期刊论文
| 作者 | Yang XY ; Liu D ; Lu P ; Zhang YJ ; Yu C |
| 刊名 | analyst
 |
| 出版日期 | 2010 |
| 卷号 | 135期号:8页码:2074-2078 |
| ISSN号 | 0003-2654 |
| 关键词 | FLUOROMETRIC DETECTION TERPYRIDYL COMPLEXES AQUEOUS-MEDIA DNA ION TELOMERE OLIGONUCLEOTIDES SEQUENCE PROBE RESISTANCE |
| 通讯作者 | yu c |
| 中文摘要 | we report a label-free fluorescence turn-on approach for the selective sensing of potassium. a properly selected g-rich oligonucleotide (oligo-y) folded into stable quadruplex structure when mixed with potassium in an aqueous solution. single-stranded nucleic acid specific nuclease was subsequently added. since an oligonucleotide in quadruplex structure is markedly more resistant to nuclease digestion than in its random coil conformation, oligo-y digestion by nuclease was considerably slow. on the other hand, oligo-y mixed with other common mono-or divalent ions was completely digested in 5 min under our experimental conditions because no quadruplex or less stable quadruplex was formed. oligo-y in potassium was subsequently mixed with a positively charged pyrene probe. electrostatic interactions between oligo-y (a polyanion) and the probe induced aggregation of the probe, which in turn induced strong pyrene excimer emission. the intensity of the induced excimer emission was directly proportional to the amount of potassium added. our method shows good sensitivity, and good selectivity against other common interference ions. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000280087000032 |
| 公开日期 | 2012-04-26 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/43590]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Yang XY,Liu D,Lu P,et al. Nucleic acid G-quadruplex based label-free fluorescence turn-on potassium selective sensing[J]. analyst,2010,135(8):2074-2078. |
| APA | Yang XY,Liu D,Lu P,Zhang YJ,&Yu C.(2010).Nucleic acid G-quadruplex based label-free fluorescence turn-on potassium selective sensing.analyst,135(8),2074-2078. |
| MLA | Yang XY,et al."Nucleic acid G-quadruplex based label-free fluorescence turn-on potassium selective sensing".analyst 135.8(2010):2074-2078. |
入库方式： OAI收割
来源：长春应用化学研究所
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