Optical Extinction Combined with Phase Measurements for Probing DNA-Small-Molecule Interactions Using an Evanescent Waveguide Biosensor
文献类型：期刊论文
| 作者 | Wang JA ; Coffey PD ; Swann MJ ; Yang F ; Lu JR ; Yang XR |
| 刊名 | analytical chemistry
 |
| 出版日期 | 2010 |
| 卷号 | 82期号:13页码:5455-5462 |
| 关键词 | SURFACE-PLASMON RESONANCE DUAL-POLARIZATION INTERFEROMETRY ANTICANCER DRUG MITOXANTRONE METHYLENE-BLUE REAL-TIME BINDING SPECTROSCOPY ADSORPTION FIELD |
| ISSN号 | 0003-2700 |
| 通讯作者 | yang xr |
| 中文摘要 | we demonstrate the use of both optical extinction and phase measurements for probing the interactions between dna and small molecules by dual polarization interferometry. on binding to dna at the interface, mitoxantrone (mtx) and methylene blue (mb) induced reversible concentration-dependent optical extinction due to light absorption, which clearly revealed the association and dissociation of small molecules with dna in real time. the binding constants of mtx dna and mb dna determined from the masses derived from optical extinction are 1.8 x 10(5) and 4.2 x 10(4) m-1, respectively, and shown to be buffer salt concentration-dependent. apart from optical extinction, phase measurements reflected the overall change of the interaction; namely, a combined result of the binding of small molecules and any changes in dna structure. the masses derived from phase could be very different from those derived from optical extinction. the structural changes detected by phase measurements showed a contraction and densification of dna upon intercalation by mtx or mb. the combination of optical extinction and phase measurements allows a detailed understanding of the interaction process. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000279253300011 |
| 公开日期 | 2012-04-26 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/43630]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Wang JA,Coffey PD,Swann MJ,et al. Optical Extinction Combined with Phase Measurements for Probing DNA-Small-Molecule Interactions Using an Evanescent Waveguide Biosensor[J]. analytical chemistry,2010,82(13):5455-5462. |
| APA | Wang JA,Coffey PD,Swann MJ,Yang F,Lu JR,&Yang XR.(2010).Optical Extinction Combined with Phase Measurements for Probing DNA-Small-Molecule Interactions Using an Evanescent Waveguide Biosensor.analytical chemistry,82(13),5455-5462. |
| MLA | Wang JA,et al."Optical Extinction Combined with Phase Measurements for Probing DNA-Small-Molecule Interactions Using an Evanescent Waveguide Biosensor".analytical chemistry 82.13(2010):5455-5462. |
入库方式： OAI收割
来源：长春应用化学研究所
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