Synthesis of a Kind of Temperature-responsive Cell Culture Surface for Corneal Sheet
文献类型：期刊论文
| 作者 | Y. Q. Guan ; Z. B. Li ; X. Wang ; X. L. Ni ; A. N. Yang ; J. M. Liu |
| 刊名 | Journal of Materials Science & Technology
 |
| 出版日期 | 2010 |
| 卷号 | 26期号:12页码:1119-1126 |
| 关键词 | N-isopropylacrylamide Low critical solution temperature Tissue engineering Corneal epithelial cells n-isopropylacrylamide stem-cells hydrogels grafts |
| ISSN号 | 1005-0302 |
| 中文摘要 | In this study, acrylic acid (AA) and 4-azidoaniline were used to modify poly (N-isopropylacrylamide) (NI-PAAm) in order to fabricate temperature-responsive surface for corneal epithelia cell adhesion and detachment. First, NIPAAm was copolymerized with acrylic acid. Then, the copolymer was coupled with azidoaniline to synthesize AzPhPIA, derivative of p(NIPAAm-co-AA), which possesses both thermo- and photo-sensitivities. Second, the synthesized copolymer was characterized by high performance liquid chromatography (HPLC), Fourier transform infrared (FTIR) and a CHN analyzer. The thermo-sensitivity was characterized by temperature reducing experiment, contact angle measurement and low critical solution temperature (LCST) testing. Third, the derivatized copolymer was immobilized by photolithography on a polystyrene plate, and then the surface characterization of AzPhPIA-coated polystyrene plate (PSt) was measured by electron spectroscopy for chemical analysis (ESCA). The thermo-sensitivity and cytocompatibility of the AzPhPIA-coated PSt were investigated by corneal epithelial cells culture. The results revealed that the AzPhPIA-coated PSt exhibited good cytocompatibility and cell detachability when temperature decreased. |
| 原文出处 | |
| 公开日期 | 2012-04-13 |
| 源URL | [http://210.72.142.130/handle/321006/31124]  |
| 专题 | 金属研究所_中国科学院金属研究所 |
| 推荐引用方式 GB/T 7714 | Y. Q. Guan,Z. B. Li,X. Wang,et al. Synthesis of a Kind of Temperature-responsive Cell Culture Surface for Corneal Sheet[J]. Journal of Materials Science & Technology,2010,26(12):1119-1126. |
| APA | Y. Q. Guan,Z. B. Li,X. Wang,X. L. Ni,A. N. Yang,&J. M. Liu.(2010).Synthesis of a Kind of Temperature-responsive Cell Culture Surface for Corneal Sheet.Journal of Materials Science & Technology,26(12),1119-1126. |
| MLA | Y. Q. Guan,et al."Synthesis of a Kind of Temperature-responsive Cell Culture Surface for Corneal Sheet".Journal of Materials Science & Technology 26.12(2010):1119-1126. |
入库方式： OAI收割
来源：金属研究所
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