Quantifying the Effects of Molecular Orientation and Length on Two-Dimensional Receptor-Ligand Binding Kinetics
文献类型：期刊论文
| 作者 | Huang J ; Chen J; Chesla SE; Yago T; Mehta P; McEver RP; Zhu C; Long M(龙勉)
|
| 刊名 | Journal of Biological Chemistry
 |
| 出版日期 | 2004 |
| 卷号 | 279期号:43页码:44915-44923 |
| 通讯作者邮箱 | cheng.zhu@me.gatech.edu ; mlong@imech.ac.cn |
| ISSN号 | 0021-9258 |
| 通讯作者 | Zhu, C (reprint author), Georgia Inst Technol, George W Woodruff Sch Mech Engn, Atlanta, GA 30332 USA. |
| 中文摘要 | Surface presentation of adhesion receptors influences cell adhesion, although the mechanisms underlying these effects are not well understood. We used a micropipette adhesion frequency assay to quantify how the molecular orientation and length of adhesion receptors on the cell membrane affected two-dimensional kinetic rates of interactions with surface ligands. Interactions of P-selectin, E-selectin, and CD16A with their respective ligands or antibody were used to demonstrate such effects. Randomizing the orientation of the adhesion receptor or lowering its ligand- and antibody-binding domain above the cell membrane lowered two-dimensional affinities of the molecular interactions by reducing the forward rates but not the reverse rates. In contrast, the soluble antibody bound with similar three-dimensional affinities to cell-bound P-selectin constructs regardless of their orientation and length. These results demonstrate that the orientation and length of an adhesion receptor influences its rate of encountering and binding a surface ligand but does not subsequently affect the stability of binding. |
| 学科主题 | 力学 |
| 类目[WOS] | Biochemistry & Molecular Biology |
| 研究领域[WOS] | Biochemistry & Molecular Biology |
| 关键词[WOS] | SELECTIN GLYCOPROTEIN LIGAND-1 ; P-SELECTIN ; CELL-ADHESION ; CONCURRENT BINDING ; MEMBRANE ; FLOW ; NEUTROPHILS ; FIBRONECTIN ; AFFINITY ; IDENTIFICATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000224505600082 |
| 公开日期 | 2007-06-15 ; 2007-12-05 ; 2009-06-23 |
| 源URL | [http://dspace.imech.ac.cn/handle/311007/15716]  |
| 专题 | 力学研究所_力学所知识产出(1956-2008) |
| 推荐引用方式 GB/T 7714 | Huang J,Chen J,Chesla SE,et al. Quantifying the Effects of Molecular Orientation and Length on Two-Dimensional Receptor-Ligand Binding Kinetics[J]. Journal of Biological Chemistry,2004,279(43):44915-44923. |
| APA | Huang J.,Chen J.,Chesla SE.,Yago T.,Mehta P.,...&龙勉.(2004).Quantifying the Effects of Molecular Orientation and Length on Two-Dimensional Receptor-Ligand Binding Kinetics.Journal of Biological Chemistry,279(43),44915-44923. |
| MLA | Huang J,et al."Quantifying the Effects of Molecular Orientation and Length on Two-Dimensional Receptor-Ligand Binding Kinetics".Journal of Biological Chemistry 279.43(2004):44915-44923. |
入库方式： OAI收割
来源：力学研究所
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