Immobilization of lipase on aminopropyl-grafted mesoporous silica nanotubes for the resolution of (R, S)-1-phenylethanol
文献类型：期刊论文
| 作者 | Bai, Wei2,3; Yang, Yun-Jie1; Tao, Xia1; Chen, Jian-Feng1; Tan, Tian-Wei2 |
| 刊名 | JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC
 |
| 出版日期 | 2012-04-01 |
| 卷号 | 76期号:doi:10.1016/j.molcatb.2011.11.005页码:82-88 |
| 关键词 | Lipase Immobilization Mesoporous silica nanotubes Aminopropyl-grafted Phenylethanol |
| 英文摘要 | Mesoporous silica nanotubes and aminopropyl-grafted mesoporous silica nanotubes were prepared as supports to immobilize lipase from Candida sp. 99-125(CILip) by physical adsorption. The immobilization conditions were investigated. Moreover, immobilized lipases on both kinds of supports were employed to catalyze olive oil hydrolization and resolution of 1-phenylethanol by esterification. The results showed that the hydrolization activity of the lipase immobilized on aminopropyl-grafted mesoporous silica nanotubes was almost twice of that on mesoporous silica nanotubes. In addition, the resolution of 1-phenylethanol catalyzed by the former catalyst also increased 22%. Circular dichroism spectra revealed a reduction of alpha-helix and an increase of beta-sheet when lipase was adsorbed on aminopropyl-grafted mesoporous silica nanotubes, which suggested that parts of alpha-helix were extended and reformed to be beta-sheet. (C) 2011 Elsevier B.V. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
| 类目[WOS] | Biochemistry & Molecular Biology ; Chemistry, Physical |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Chemistry |
| 关键词[WOS] | ENZYME IMMOBILIZATION ; CATALYTIC-PROPERTIES ; NANOPOROUS SUPPORT ; SBA-15 ; ADSORPTION ; SURFACES |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000301033300012 |
| 公开日期 | 2011-12-28 |
| 源URL | [http://localhost/handle/0/199]  |
| 专题 | 天津工业生物技术研究所_功能糖与天然活性物质研究组 孙媛霞 _期刊论文 |
| 作者单位 | 1.Beijing Univ Chem Technol, Minist Educ, Key Lab Nanomat, Beijing 100029, Peoples R China 2.Beijing Univ Chem Technol, Coll Life Sci & Technol, Beijing Key Lab Bioproc, Beijing 100029, Peoples R China 3.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin 300308, Peoples R China |
| 推荐引用方式 GB/T 7714 | Bai, Wei,Yang, Yun-Jie,Tao, Xia,et al. Immobilization of lipase on aminopropyl-grafted mesoporous silica nanotubes for the resolution of (R, S)-1-phenylethanol[J]. JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC,2012,76(doi:10.1016/j.molcatb.2011.11.005):82-88. |
| APA | Bai, Wei,Yang, Yun-Jie,Tao, Xia,Chen, Jian-Feng,&Tan, Tian-Wei.(2012).Immobilization of lipase on aminopropyl-grafted mesoporous silica nanotubes for the resolution of (R, S)-1-phenylethanol.JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC,76(doi:10.1016/j.molcatb.2011.11.005),82-88. |
| MLA | Bai, Wei,et al."Immobilization of lipase on aminopropyl-grafted mesoporous silica nanotubes for the resolution of (R, S)-1-phenylethanol".JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC 76.doi:10.1016/j.molcatb.2011.11.005(2012):82-88. |
入库方式： OAI收割
来源：天津工业生物技术研究所
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