Cloning, expression, and characterization of a thermostable glucoamylase from Thermoanaerobacter tengcongensis MB4
文献类型：期刊论文
| 作者 | Zheng, Yingying1,2; Xue, Yanfen1; Zhang, Yueling3; Zhou, Cheng1; Schwaneberg, Ulrich4; Ma, Yanhe1 |
| 刊名 | APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
 |
| 出版日期 | 2010-06-01 |
| 卷号 | 87期号:1页码:225-233 |
| 关键词 | Glucoamylase Thermoanaerobacter tengcongensis Recombinant expression Thermostable |
| 英文摘要 | A thermostable glucoamylase (TtcGA) from Thermoanaerobacter tengcongensis MB4 was successfully expressed in Escherichia coli. The full-length gene (2112 bp) encodes a 703-amino acid polypeptide including a predicted signal peptide of 21 residues. The recombinant mature protein was partially purified to 30-fold homogeneity by heat treatment and gel filtration chromatography. The mature protein is a monomer with the molecular weight of 77 kD. The recombinant enzyme showed maximum activity at 75 A degrees C and pH 5.0. It is the most thermostable bacterial glucoamylase described to date with nearly no activity loss after incubation at 75 A degrees C for 6 h. TtcGA can hydrolyze both alpha-1, 4- and alpha-1, 6-glycosidic linkages in various alpha-glucans. It showed preference for maltooligosaccharides over polysaccharides with specific activity of 80 U/mg towards maltose. Kinetic studies revealed that TtcGA had the highest activity on maltooligosaccharide with four monosaccharide units. The cations Ca(2+), Mn(2+), Co(2+), Mg(2+), and reducing agent DTT showed no obvious effects on the action of TtcGA. In contrast, the enzyme was inactivated by Zn(2+), Pb(2+), Cu(2+), and EDTA. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biotechnology & Applied Microbiology |
| 研究领域[WOS] | Biotechnology & Applied Microbiology |
| 关键词[WOS] | BIOCHEMICAL-CHARACTERIZATION ; THERMOACTIVE GLUCOAMYLASE ; THERMOPLASMA-ACIDOPHILUM ; CRYSTAL-STRUCTURE ; SIGNAL PEPTIDES ; PURIFICATION ; CLOSTRIDIUM ; SEQUENCE ; THERMOSACCHAROLYTICUM ; ENZYME |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000277784100023 |
| 公开日期 | 2011-08-15 |
| 源URL | [http://localhost/handle/0/75]  |
| 专题 | 天津工业生物技术研究所_基因工程与微生物应用技术研究组 宋恢_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, State Key Lab Microbial Resources, Inst Microbiol, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin 300308, Peoples R China 4.Rhein Westfal TH Aachen, Lehrstuhl Biotechnol, D-52074 Aachen, Germany |
| 推荐引用方式 GB/T 7714 | Zheng, Yingying,Xue, Yanfen,Zhang, Yueling,et al. Cloning, expression, and characterization of a thermostable glucoamylase from Thermoanaerobacter tengcongensis MB4[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2010,87(1):225-233. |
| APA | Zheng, Yingying,Xue, Yanfen,Zhang, Yueling,Zhou, Cheng,Schwaneberg, Ulrich,&Ma, Yanhe.(2010).Cloning, expression, and characterization of a thermostable glucoamylase from Thermoanaerobacter tengcongensis MB4.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,87(1),225-233. |
| MLA | Zheng, Yingying,et al."Cloning, expression, and characterization of a thermostable glucoamylase from Thermoanaerobacter tengcongensis MB4".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 87.1(2010):225-233. |
入库方式： OAI收割
来源：天津工业生物技术研究所
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