Enhancement of dihydroxyacetone production by a mutant of Gluconobacter oxydans
文献类型：期刊论文
| 作者 | Lijuan Ma ; Wenyu Lu ; Zhendong Xia ; Jianping Wen |
| 刊名 | Biochemical Engineering Journal
 |
| 出版日期 | 2010-03-15 |
| 卷号 | 49期号:1页码:61-67 |
| 关键词 | He–Ne laser irradiation Bioconversion 1,3-Dihydroxyacetone Glycerol Enzyme activity Kinetics |
| 英文摘要 | To improve the yield of 1,3-dihydroxyacetone(DHA) and the bioconversion rate of glycerol by Gluconobacter oxydans(G. oxydans), He-Ne laser irradiation technology was employed in this work. Under the optimal irradiation doses of 21 mW, 21 min, mutant G. oxydans GM51 with high DHAproduction capacity and stable heredity was obtained through tolerance tests to glycerol and DHA. Activity of the key enzyme, glycerol dehydrogenase, in GM51 was 5.01 U/mL after 30h culture, 75.17% higher than that in the wild (2.86 U/mL) with the same initial glycerol concentration of 100 g/L The culture time of GM51 had been shortened by 16 h (conventional culture time is 58 h). DHA yield of CM51 was up to 91.5%, increased by 77.6% with an improvement of DHA productivity from 1.29 to 2.29 g L(-1) h(-1) when cultivated in 7 L bioreactor. Moreover, comparative studies on kinetics of the mutantand wild further confirmed the decrease in inhibitory of substrate and product through He-Ne laser irradiation. The mutagenesis mechanism was speculated as the mutation of the gene encoding glycerol dehydrogenase, which led to improvement in the activity of this enzyme. Therefore, He-Ne laser irradiation could efficiently enhance the DHA production ability of G. oxydans. (C) 2009 Elsevier B.V. All rights reserved. |
| 公开日期 | 2011-08-15 |
| 源URL | [http://localhost/handle/0/71]  |
| 专题 | 天津工业生物技术研究所_工业生物系统工程实验室 陈树林_期刊论文 |
| 推荐引用方式 GB/T 7714 | Lijuan Ma,Wenyu Lu,Zhendong Xia,等. Enhancement of dihydroxyacetone production by a mutant of Gluconobacter oxydans[J]. Biochemical Engineering Journal,2010,49(1):61-67. |
| APA | Lijuan Ma,Wenyu Lu,Zhendong Xia,&Jianping Wen.(2010).Enhancement of dihydroxyacetone production by a mutant of Gluconobacter oxydans.Biochemical Engineering Journal,49(1),61-67. |
| MLA | Lijuan Ma,et al."Enhancement of dihydroxyacetone production by a mutant of Gluconobacter oxydans".Biochemical Engineering Journal 49.1(2010):61-67. |
入库方式： OAI收割
来源：天津工业生物技术研究所
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